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ATG14 Antibody (PA5-85457) in WB
Western blot analysis of ATG14 in wild-type (WT) and ATG14 knockout (KO) HeLa cell extracts using ATG14 polyclonal antibody (Product # PA5-85457) using 30 µg of sample at a dilution of 1:3000. Sample was then incubated with HRP-conjugated anti-rabbit IgG and ECL secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
Product Details
PA5-85457
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG14.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792597
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (93%), Rat (91%), Bovine (94%).
Positive Control: Huh7, HeLa.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG14 (Autophagy related protein homolog 14) is encoded by the ATG14 gene and is located on chromosome 14 in humans. It is also known as ATG14L or BARKOR (Beclin 1-associated autophagy-related key regulator). Autophagy is an evolutionarily conserved process that involves recycling of misfolded cellular proteins and degradation of dysfunctional organelles. Autophagy is induced as a cellular response to nutrient stress which includes the formation of autophagosomes, fusion of these with the lysosome and formation of the autophagolysosome. The Class III PI3-Kinase, Vps34 and its interacting partner Beclin 1 have been shown to form a complex which specifically includes ATG 14 (type 1) or UVRAG (type 2). Atg 14 guides the type 1 complex to the preautophagosomal structure (PAS) and is critical for the initiation of autophagosome formation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG14 autophagy related 14 homolog; Atg14L; Autophagy-related protein 14-like protein; Bakor; Barkor; Beclin 1-associated autophagy-related key regulator; Beclin 1-Interacting protein
Gene Aliases: ATG14; ATG14L; BARKOR; KIAA0831
UniProt ID: (Human) Q6ZNE5
Entrez Gene ID: (Human) 22863
autophagosome assembly
regulation of protein phosphorylation
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
endosome to lysosome transport
cellular response to starvation
positive regulation of autophagy
posttranscriptional regulation of gene expression
macroautophagy
cellular response to glucose starvation
positive regulation of phosphatidylinositol 3-kinase activity
regulation of protein complex stability
regulation of triglyceride metabolic process
mitophagy in response to mitochondrial depolarization
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