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Invitrogen
ATG4A Monoclonal Antibody (514914)
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Application
FIGURE: 1 / 1
ATG4A Antibody (MA5-24225) in ICC/IF
Immunocytochemistry analysis of ATG4A in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line. Samples were incubated in ATG4A monoclonal antibody (Product # MA5-24225) using a dilution of 25 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
MA5-24225
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
514914
Immunogen
Sf 21-derived recombinant human ATG4A isoform 1 Glu2-Val398
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606321
Product Specific Information
In direct ELISAs and Western blots, no cross-reactivity with recombinant human ATG4B is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG4 (ATG4) autophagy-related homolog A; APG4 autophagy 4 homolog A; ATG4 autophagy related 4 homolog A; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; autophagin 2; Autophagin-2; autophagy 4 homolog A; autophagy related 4A, cysteine peptidase; autophagy-related 4A; Autophagy-related cysteine endopeptidase 2; Autophagy-related protein 4 homolog A; cysteine protease APG4A; Cysteine protease ATG4A; hAPG4A; HsAPG4A; MGC107179
Gene Aliases: AI627006; APG4A; ATG4A; Atg4al; AUTL2; AV169859
UniProt ID: (Human) Q8WYN0, (Mouse) Q8C9S8
Entrez Gene ID: (Human) 115201, (Mouse) 666468
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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