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Application
FIGURE: 1 / 1
ATG4B Antibody (PA5-86908) in WB
Western blot analysis of ATG4B in Lane 1: HCT116 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: Kidney lysate of mouse (40 µg), Lane 4: Kidney lysate of rat (40 µg). Sample was incubated with ATG4B polyclonal antibody (Product # PA5-86908) using a 1:500 dilution.
Product Details
PA5-86908
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 75-119 of Human ATG4B
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2803666
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; Autophagin-1; autophagy related 4 homolog B; autophagy related 4B, cysteine peptidase; autophagy-related 4B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; HsAPG4B
Gene Aliases: APG4B; ATG4B; AUTL1; KIAA0943
UniProt ID: (Human) Q9Y4P1
Entrez Gene ID: (Human) 23192, (Rat) 316640
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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