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            • Primary Antibodies ›
            • ATG4B Antibodies

            Invitrogen

            ATG4B Polyclonal Antibody

            View all (29) ATG4B antibodies
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            Cite ATG4B Polyclonal Antibody

            • Antibody Testing Data (1)
            Application
            ATG4B Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            ATG4B Antibody (PA5-86908) in WB

            Western blot analysis of ATG4B in Lane 1: HCT116 whole cell lysate (40 µg), Lane 2: HEK293T whole cell lysate (40 µg), Lane 3: Kidney lysate of mouse (40 µg), Lane 4: Kidney lysate of rat (40 µg). Sample was incubated with ATG4B polyclonal antibody (Product # PA5-86908) using a 1:500 dilution.
            View Product

            ATG4B Antibody in Western Blot (WB)

            Product Details

            PA5-86908

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:500-1:1,000
            -
            Product Specifications

            Species Reactivity

            Human, Rat

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Synthetic peptide, corresponding to amino acids 75-119 of Human ATG4B
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.2, with 50% glycerol

            Contains

            0.02% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2803666

            Product Specific Information

            Purity is > 95% by SDS-PAGE.

            Target Information

            Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Cite this product

            Bioinformatics

            Protein Aliases: APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; Autophagin-1; autophagy related 4 homolog B; autophagy related 4B, cysteine peptidase; autophagy-related 4B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; HsAPG4B

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            Gene Aliases: APG4B; ATG4B; AUTL1; KIAA0943

            View more View less

            UniProt ID: (Human) Q9Y4P1

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            Entrez Gene ID: (Human) 23192, (Rat) 316640

            View more View less

            Function(s)

            endopeptidase activity cysteine-type endopeptidase activity protein binding cysteine-type peptidase activity molecular_function peptidase activity cysteine protease

            Process(es)

            autophagosome assembly mitophagy C-terminal protein lipidation proteolysis protein targeting to membrane autophagy macroautophagy protein processing nucleophagy positive regulation of protein catabolic process protein delipidation cellular response to nitrogen starvation positive regulation of autophagy
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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