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Application
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ATG4C Antibody (MA5-56428) in WB
Western blot analysis of ATG4C in Lane 1: 293T cell lysate (20 µg/Lane), Lane 2: Jurkat cell lysate (20 µg/Lane), Lane 3: Raji cell lysate (20 µg/Lane), Lane 4: A431 cell lysate (20 µg/Lane), Lane 5: HeLa cell lysate (20 µg/Lane), Lane 6: K-562 cell lysate (20 µg/Lane), Lane 7: HL-60 cell lysate (20 µg/Lane), Lane 8: Mouse brain tissue lysate (40 µg/Lane), Lane 9: Rat brain tissue lysate (40 µg/Lane), Lane 10: COS-1 cell lysate (20 µg/Lane). Samples were incubated with ATG4C Recombinant Monoclonal Antibody (Product # MA5-56428) using a dil... View More
Product Details
MA5-56428
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH05-23
Immunogen
Recombinant protein within human ATG4C aa 1-458/458.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3679483
Target Information
ATG4C is involved in autophagy, the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding the same protein, have been characterized. Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Highly expressed in skeletal muscle, heart, liver and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG4 (ATG4) autophagy-related homolog C; APG4 autophagy 4 homolog C; ATG4 autophagy related 4 homolog C; AUT-like 1, cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; autophagin 3; Autophagin-3; autophagy related 4C, cysteine peptidase; autophagy-related 4C; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; cysteine peptidase; Cysteine protease ATG4C; cysteine protease involved in autophagy; FLJ14867; HsAPG4C
Gene Aliases: APG4-C; APG4C; ATG4C; Atg4cl; AUTL1; AUTL3
UniProt ID: (Human) Q96DT6, (Mouse) Q811C2
Entrez Gene ID: (Human) 84938, (Mouse) 242557, (Rat) 313391
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