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            • Primary Antibodies ›
            • alpha Tubulin Antibodies

            Invitrogen

            Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)

            Advanced Verification
            34 Published Figures
            73 References
            View all (89) alpha Tubulin antibodies

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            Datasheet
            Protocols
            Questions & Answers
            Catalog # 32-2700
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            Price (USD)
            514.00
            100 µg
             
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            Cite Acetyl-alpha Tubulin (Lys40) Monoclonal Antibody (6-11B-1)

            • Antibody Testing Data (4)
            • Published Figures (34)
            • Advanced Verification (2)
            Application
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Group 53 Created with Sketch.

            FIGURE: 1 / 40

            Acetyl-alpha Tubulin (Lys40) Antibody (32-2700) in ICC/IF

            Immunofluorescent analysis of Alpha-Tubulin (Acetylated) was done on 70% confluent log phase HeLa cells treated with 5mM sodium butyrate for 24 h. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Alpha-Tubulin (Acetylated) Recombinant Mouse Monoclo... View More
            View Product

            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Flow Cytometry (Flow)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry (ICC/IF)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Immunohistochemistry (IHC)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody in Western Blot (WB)
            Acetyl-alpha Tubulin (Lys40) Antibody
            Acetyl-alpha Tubulin (Lys40) Antibody

            Product Details

            32-2700

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1-3 µg/mL
            View 10 publications 10 publications

            Immunohistochemistry (IHC)

            -
            View 23 publications 23 publications

            Immunohistochemistry (Paraffin) (IHC (P))

            -
            View 1 publication 1 publication

            Immunohistochemistry (Frozen) (IHC (F))

            -
            View 1 publication 1 publication

            Immunocytochemistry (ICC/IF)

            1-3 µg/mL
            View 36 publications 36 publications

            Flow Cytometry (Flow)

            Assay-dependent
            -

            Miscellaneous PubMed (Misc)

            -
            View 2 publications 2 publications
            Product Specifications

            Species Reactivity

            Human, Mouse, Rat, Sea urchin

            Published species

            Algae, Arthropod, Chicken, Dog, Flatworm, Human, Mollusc, Mouse, Protozoa, Rat, Zebrafish

            Host/Isotype

            Mouse / IgG2b

            Class

            Monoclonal

            Type

            Antibody

            Clone

            6-11B-1

            Immunogen

            Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Protein A

            Storage buffer

            PBS, pH 7.4

            Contains

            0.1% sodium azide

            Storage conditions

            -20°C

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2533073

            Product Specific Information

            This antibody reacts with the ~ 55 kDa acetylated form of alpha-tubulin. This antibody reacts with human, mouse, and rat. Reactivity is confirmed with NIH3T3 and HeLa cells.

            Target Information

            Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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            Bioinformatics

            Protein Aliases: alpha tubulin; Alpha-tubulin 1; Alpha-tubulin 2; Alpha-tubulin 3; Alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3E; Alpha-tubulin 4; Alpha-tubulin 6; Alpha-tubulin isotype M-alpha-1; Alpha-tubulin isotype M-alpha-2; Alpha-tubulin isotype M-alpha-4; Alpha-tubulin isotype M-alpha-6; Alpha-tubulin ubiquitous; beta-tubulin T beta15 (aa 1-445); class II beta-tubulin isotype; class IIb beta-tubulin; FLJ30169; H2 ALPHA; hum-a-tub1; hum-a-tub2; T beta-15; Talpha1 alpha-tubulin; Testis-specific alpha-tubulin; TUBA1/3/4; Tubulin alpha; Tubulin alpha chain-like 3; Tubulin alpha-1 chain; Tubulin alpha-1A chain; Tubulin alpha-1B chain; Tubulin alpha-1C chain; Tubulin alpha-2 chain; Tubulin alpha-3 chain; Tubulin alpha-3C chain; Tubulin alpha-3E chain; Tubulin alpha-4 chain; Tubulin alpha-4A chain; Tubulin alpha-6 chain; Tubulin alpha-ubiquitous chain; Tubulin B-alpha-1; Tubulin beta-2B chain; Tubulin H2-alpha; Tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 1b; tubulin, alpha 1c; tubulin, alpha 2; tubulin, alpha 3c; tubulin, alpha 3e; tubulin, alpha 4; tubulin, alpha 4a; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, alpha-like 3; tubulin, beta 2; tubulin, beta 2B class IIb; tubulin, beta polypeptide paralog

            View more View less

            Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; PMGYSA; RGD1309427; RGD1565476; Tuba-1; TUBA1; TUBA1A; TUBA1B; TUBA1C; TUBA2; TUBA3; TUBA3C; TUBA3E; Tuba4; TUBA4A; TUBA6; TUBAL3; Tubb2; Tubb2a; TUBB2B

            View more View less

            UniProt ID: (Human) Q71U36, (Human) P68363, (Human) Q9BQE3, (Human) Q6PEY3, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) A8K068, (Mouse) P68369, (Rat) P68370, (Mouse) P05213, (Rat) Q6P9V9, (Mouse) P68373, (Rat) Q6AYZ1, (Mouse) P68368, (Rat) Q5XIF6, (Mouse) Q3UX10, (Mouse) Q9CWF2, (Rat) Q3KRE8

            View more View less

            Entrez Gene ID: (Human) 7846, (Human) 10376, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Human) 79861, (Human) 347733, (Mouse) 22142, (Rat) 64158, (Mouse) 22143, (Rat) 500929, (Mouse) 22146, (Rat) 300218, (Mouse) 22145, (Rat) 316531, (Rat) 291287, (Mouse) 238463, (Mouse) 73710, (Rat) 291081

            View more View less

            Function(s)

            GTPase activity structural molecule activity structural constituent of cytoskeleton protein binding GTP binding protein domain specific binding double-stranded RNA binding ubiquitin protein ligase binding molecular_function enzyme binding nucleotide binding protein heterodimerization activity tubulin

            Process(es)

            G2/M transition of mitotic cell cycle cytoskeleton organization microtubule-based process cytoskeleton-dependent intracellular transport cell division microtubule cytoskeleton organization cellular response to interleukin-4 biological_process platelet degranulation neuron migration protein polymerization metabolic process
            It has to be done as per old AB suggested Products section.
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