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Invitrogen
Acrolein Monoclonal Antibody (10A10)
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Application
FIGURE: 1 / 4
Acrolein Antibody (MA5-27553) in ICC/IF
Immunofluorescent analysis of Acrolein in human embryonic kidney cells (HEK293). Sample was fixed with 5% formaldehyde (5 min), incubated with Acrolein monoclonal antibody (Product # MA5-27553) using a dilution of 1:50 (30-60 min at RT), and followed by Goat Anti-Mouse Alexa Fluor 488, Phalloidin Alexa Fluor 633 and DAPI secondary antibody at a dilution of 1:1500, 1:250 and 1:50,000 (30-60 min at RT). Images are shown as follows: (A,C,E,G) - Untreated, (B,D,F,H) - Cells cultured overnight with 50 µM H2O2, (A,B) DAPI (blue) nuclear stain, (... View More
Product Details
MA5-27553
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chemical
Published species
Chemical
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10A10
Immunogen
Synthetic Acrolein modified Keyhole Limpet Hemocyanin (KLH)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735099
Product Specific Information
A 1:1,000 dilution of MA5-27553 was sufficient for detection of Acrolein in 2 µg of Acrolein conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Acrolein modified proteins. Does not detect free acrolein. Does not cross-react with Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
Target Information
Acrolein is primarily used as an intermediate in the synthesis of acrylic acid and as a biocide. Acrolein may be formed from the breakdown of certain pollutants in outdoor air or from the burning of organic matter including tobacco, or fuels such as gasoline or oil. Acrolein is toxic to humans following inhalation, oral or dermal exposures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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