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Invitrogen

Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)

Name: Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)
Brand: Invitrogen
SKU: MA191637
Price: 550.0 USD
Availability: InStock

Advanced Verification

7 Published Figures

11 References

View all (5) Active/Pro-Caspase 3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

Active/Pro-Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Active/Pro-Caspase 3 Antibody (MA1-91637) in IHC (P)

Immunohistochemistry (Paraffin) analysis of Caspase 3 (active/pro) in human thymus tissue using Caspase 3 (active/pro) Monoclonal Antibody (31A1067) (Product # MA1-91637) at a dilution of 5 µg/mL. Antigen retrieval : Heat-induced antigen retrieval.

Active/Pro-Caspase 3 Antibody in Western Blot (WB)

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Product Details

MA1-91637

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

Immunomicroscopy (IM)

Assay-dependent

Mass Spectrometry (MS)

Assay-dependent

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Pig, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

31A1067

Immunogen

Full-length recombinant human caspase-3 protein was used as immunogen. The antibody recognizes an epitope in the large domain subunit of Caspase-3. As such it will recognize pro Caspase-3 and the large subunit cleavage fragment.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1954999

Product Specific Information

Predicted molecular weight: 31kDa.

Positive Control: Staurosporine-treated HeLa or Jurkat cell lysate.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thyroid hormone-induced cell death in sea urchin metamorphic development.

The Journal of experimental biology

Wynen H,Taylor E,Heyland A

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Immunohistochemistry (Paraffin)

Thu Dec 01 00:00:00 UTC 2022

The protective effects of propofol against renal ischemia-reperfusion injury are potentiated by norisoboldine treatment via inhibition of oxidative stress pathways.

Journal of biochemical and molecular toxicology

Xing D,Li Q,Lin G,Lin H,Kang W,Zhang M,Ding R,Li N

MA1-91637 was used in Western Blotting to suggest that treatment with NOR potentiated the nephroprotective effects of propofol in rats with I/R-induced renal injury by ameliorating oxidative stress and apoptosis pathway.

Sat Jan 01 00:00:00 UTC 2022

Chemotherapy impairs skeletal muscle mitochondrial homeostasis in early breast cancer patients.

Journal of cachexia, sarcopenia and muscle

Mallard J,Hucteau E,Charles AL,Bender L,Baeza C,Pélissie M,Trensz P,Pflumio C,Kalish-Weindling M,Gény B,Schott R,Favret F,Pivot X,Hureau TJ,Pagano AF

MA1-91637 was used in Western Blotting to investigating overall skeletal muscle mitochondrial homeostasis in early breast cancer patients undergoing chemotherapy, including mitochondrial quantity, function, and dynamics.

Wed Jun 01 00:00:00 UTC 2022

Aging lens epithelium is susceptible to ferroptosis.

Free radical biology & medicine

Wei Z,Hao C,Huangfu J,Srinivasagan R,Zhang X,Fan X

MA1-91637 was used in Western Blotting to explore the lens epithelial cell's (LEC) susceptibility to ferroptosis after documentation has concluded that aged and cataractous human lenses manifest with increased reactive oxygen species (ROS) formation, elevated lipid peroxidation, and accumulative intracellular redox-active iron, constituting the three hallmarks of ferroptosis during aging and cataractogenesis.

Sat May 01 00:00:00 UTC 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Western Blot

Wed Dec 01 00:00:00 UTC 2021

Mitotic Activation Around Wound Edges and Epithelialization Repair in UVB-Induced Capsular Cataracts.

Investigative ophthalmology & visual science

Wei Z,Hao C,Srinivasagan R,Wu H,Chen JK,Fan X

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Western Blot

Wed Dec 01 00:00:00 UTC 2021

Pristimerin inhibits neuronal inflammation and protects cognitive function in mice with sepsis-induced brain injuries by regulating PI3K/Akt signalling.

Pharmaceutical biology

Xue W,Li Y,Zhang M

MA1-91637 was used in Western Blot to explore whether pristimerin protected against cognitive dysfunction and neuroinflammation in C57BL/6 J mice with sepsis-induced brain injuries.

Wed Dec 01 00:00:00 UTC 2021

Pristimerin inhibits neuronal inflammation and protects cognitive function in mice with sepsis-induced brain injuries by regulating PI3K/Akt signalling.

Pharmaceutical biology

Xue W,Li Y,Zhang M

MA1-91637 was used in Western Blot to explore whether pristimerin protected against cognitive dysfunction and neuroinflammation in C57BL/6 J mice with sepsis-induced brain injuries.

Wed Dec 01 00:00:00 UTC 2021

β-arrestin 2 Is a Prognostic Factor for Survival of Ovarian Cancer Patients Upregulating Cell Proliferation.

Frontiers in endocrinology

Czogalla B,Partenheimer A,Jeschke U,von Schönfeldt V,Mayr D,Mahner S,Burges A,Simoni M,Melli B,Benevelli R,Bertini S,Casarini L,Trillsch F

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Western Blot

Wed May 26 00:00:00 UTC 2021

β-arrestin 2 Is a Prognostic Factor for Survival of Ovarian Cancer Patients Upregulating Cell Proliferation.

Frontiers in endocrinology

Czogalla B,Partenheimer A,Jeschke U,von Schönfeldt V,Mayr D,Mahner S,Burges A,Simoni M,Melli B,Benevelli R,Bertini S,Casarini L,Trillsch F

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Western Blot

Wed May 26 00:00:00 UTC 2021

Therapeutic effect of Resveratrol in the treatment of osteoarthritis via the MALAT1/miR-9/NF-κB signaling pathway.

Experimental and therapeutic medicine

Zhang G,Zhang H,You W,Tang X,Li X,Gong Z

Published figure using Active/Pro-Caspase 3 monoclonal antibody (Product # MA1-91637) in Western Blot

Sun Mar 01 00:00:00 UTC 2020

Estrogen Modulates Specific Life and Death Signals Induced by LH and hCG in Human Primary Granulosa Cells In Vitro.

International journal of molecular sciences

Casarini L,Riccetti L,De Pascali F,Gilioli L,Marino M,Vecchi E,Morini D,Nicoli A,La Sala GB,Simoni M

MA1-91637 was used in Western Blotting to study the pro- and anti-apoptotic effect of leutenising hormone and human chorionic gonadotrophin in the presence or absence of oestrogen.

Fri Apr 28 00:00:00 UTC 2017

Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1.

Molecular cancer

Arora R,Sawney S,Saini V,Steffi C,Tiwari M,Saluja D

Published figure using Caspase 3 (active/pro) monoclonal antibody (Product # MA1-91637) in Western Blot

Tue Oct 18 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Apopain; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; CPP-32; CPP32beta; Cysteine protease CPP32; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

UniProt ID: (Human) P42574, (Pig) Q95ND5, (Mouse) P70677, (Rat) P55213

Entrez Gene ID: (Human) 836, (Pig) 397244, (Chinese hamster) 100689061, (Mouse) 12367, (Rat) 25402

Function(s)

protease binding aspartic-type endopeptidase activity cysteine-type endopeptidase activity cyclin-dependent protein serine/threonine kinase inhibitor activity death receptor binding protein binding peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process phospholipase A2 activator activity protein complex binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in execution phase of apoptosis cysteine-type endopeptidase activity involved in apoptotic signaling pathway cysteine-type peptidase activity hydrolase activity protease

Process(es)

response to hypoxia B cell homeostasis apoptotic DNA fragmentation proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cellular response to DNA damage stimulus heart development sensory perception of sound learning or memory activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c response to UV response to glucose response to X-ray regulation of macroautophagy protein processing hippocampus development neuron differentiation keratinocyte differentiation erythrocyte differentiation platelet formation negative regulation of B cell proliferation response to cobalt ion response to estradiol response to lipopolysaccharide glial cell apoptotic process response to tumor necrosis factor response to nicotine hippo signaling wound healing response to drug response to hydrogen peroxide T cell homeostasis negative regulation of apoptotic process response to amino acid positive regulation of neuron apoptotic process cell fate commitment negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of activated T cell proliferation response to antibiotic neurotrophin TRK receptor signaling pathway response to glucocorticoid neuron apoptotic process cellular response to organic cyclic compound apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand execution phase of apoptosis viral process regulation of protein stability positive regulation of beta-amyloid formation response to wounding intracellular signal transduction positive regulation of apoptotic process positive regulation of catalytic activity negative regulation of cell cycle cellular response to organic substance release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to oxidative stress response to organic substance response to metal ion response to organic cyclic compound regulation of apoptotic DNA fragmentation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Active/Pro-Caspase 3 Monoclonal Antibody (31A1067)

Active/Pro-Caspase 3 Antibody (MA1-91637) in IHC (P)

Product Details

MA1-91637

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunomicroscopy (IM)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions