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            • Primary Antibodies ›
            • ADORA2B Antibodies

            Alomone Labs, LTD

            Adenosine A2B Receptor (extracellular) Polyclonal Antibody, FITC

            View all (13) ADORA2B antibodies
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            Catalog # AAR-003-F-50UL
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            Price (USD)
            900.00
            50 µL
             
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            Cite Adenosine A2B Receptor (extracellular) Polyclonal Antibody, FITC

            • Antibody Testing Data (2)
            Application
            Adenosine A2B Receptor (extracellular) Antibody in Flow Cytometry (Flow)
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            Adenosine A2B Receptor (extracellular) Antibody (AAR-003-F-50UL) in Flow

            Cell surface detection of ADORA2b in live intact human THP-1 monocytic leukemia cells: - (black) Cells alone. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + AdenosineA2BReceptor (extracellular)-FITC Antibody (#AAR-003-F), (5 µg).
            View Product

            Adenosine A2B Receptor (extracellular) Antibody in Flow Cytometry (Flow)
            Adenosine A2B Receptor (extracellular) Antibody in Flow Cytometry (Flow)

            Product Details

            AAR-003-F-50UL

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            5 µg
            -
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Rabbit / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            KDSATNN(S)TEPWDGTTNESC, corresponding to amino acid residues 147-166 of humanA2BAR, 2nd extracellular loop
            View immunogen

            Conjugate

            FITC

            Excitation/Emission Max

            498/517 nm View spectra spectra

            Form

            Lyophilized

            Concentration

            1 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS, pH 7.4, with 1% BSA

            Contains

            0.05% sodium azide

            Storage conditions

            -20°C, Avoid Freeze/Thaw Cycles, store in dark

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).

            Target Information

            ADORA2B encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
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            Bioinformatics

            Protein Aliases: A2b receptor; A2b, Rs; Adenosine receptor A2b; HGNC:264; netrin-1 receptor

            View more View less

            Gene Aliases: A2b; A2BAR; A2BR; AA2BR; ADORA2; ADORA2B; AI480866; AI605384

            View more View less

            UniProt ID: (Human) P29275, (Mouse) Q60614

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            Entrez Gene ID: (Human) 136, (Mouse) 11541

            View more View less

            Function(s)

            G-protein coupled adenosine receptor activity signal transducer activity G-protein coupled receptor activity G-protein coupled receptor

            Process(es)

            activation of MAPK activity adenosine receptor signaling pathway positive regulation of chronic inflammatory response to non-antigenic stimulus cellular defense response G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway activation of adenylate cyclase activity JNK cascade excretion positive regulation of vascular endothelial growth factor production positive regulation of guanylate cyclase activity cellular response to extracellular stimulus positive regulation of chemokine production positive regulation of interleukin-6 production positive regulation of mast cell degranulation cellular protein metabolic process relaxation of vascular smooth muscle signal transduction positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of norepinephrine secretion positive regulation of steroid biosynthetic process positive regulation of cAMP biosynthetic process positive regulation of cGMP biosynthetic process negative regulation of collagen biosynthetic process positive regulation of catecholamine secretion regulation of angiogenesis positive regulation of vasodilation
            It has to be done as per old AB suggested Products section.
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