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Alomone Labs, LTD
Adenosine A3 Receptor (extracellular) Polyclonal Antibody, FITC
FIGURE: 1 / 2
Adenosine A3 Receptor (extracellular) Antibody (AAR-035-F-50UL) in Flow
Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse WEHI-231 B lymphocyte cell line: - (black line) cells. (red) Cells + Rabbit IgG Isotype Control-FITC. (green) Cells + Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F), (2.5 µg).
Product Details
AAR-035-F-50UL
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)GWNRKATLASSQN, corresponding to amino acid residues 149-161 of mouse ADORA3, Extracellular, 2nd loop
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 15 µL or 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
Adenosine is involved in a variety of processes, including the synthesis of urea, the anti-inflammatory response and the inhibition of protein synthesis The adenosine receptors, including adenosine A1-R, adenosine A2A-R, adenosine A2B-R and adenosine A3-R, are integral membrane proteins that are members of the G protein-coupled receptor family. The A1-R protein mediates ureagenesis in a partially calcium-dependent manner. Adenosine is known to mediate coronary vasodilation via the A2A-R receptor. Collagen synthesis and total protein synthesis are inhibited in certain cells by adenosine, acting via the A2B receptors. Activation of the A3-R receptor inhibits the induction of the cytokine TNF alpha and blocks the endotoxin CD14 receptor signal transduction pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AD026; Adenosine A3-R; adenosine receptor A3; BA552M11.5; G-protein coupled receptor 2; RP11-552M11.7
Gene Aliases: A3AR; A3R; AA3R; ARA3; Gpcr2; Tgpcr1
Entrez Gene ID: (Mouse) 11542, (Rat) 25370
adenosine receptor signaling pathway
histamine secretion by mast cell
positive regulation of leukocyte migration
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of mast cell degranulation
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of mucus secretion
G-protein coupled receptor signaling pathway
regulation of norepinephrine secretion
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