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Chromotek
Alpaca anti-Human IgG/Rabbit IgG VHH, Nano-Secondaries™ Recombinant Secondary Antibody, Alexa Fluor™ 568
Application
FIGURE: 1 / 3
Human IgG/Rabbit IgG VHH Secondary Antibody (SRBAF568-1-100) in ICC/IF
HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Product Details
SRBAF568-1-100
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:1,000
Product Specifications
Species Reactivity
Human,
Rabbit
Host/Isotype
Alpaca
Expression System
E. coli
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
CTK0101, CTK0102
Immunogen
rabbit IgG
Conjugate
Excitation/Emission Max
579/603 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
10mM HEPES, pH 7, with 5mM EDTA, 500mM NaCl
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target
IgG
Antibody Form
VHH
Product Specific Information
Anti-human IgG and rabbit IgG Nanobody for detection of human IgG and rabbit IgG in Immunofluorescence and Western Blot.
Affinity: CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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