Invitrogen

alpha Actinin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

9 References

View all (18) alpha Actinin 1 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

alpha Actinin 1 Antibody in Immunocytochemistry (ICC/IF)

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alpha Actinin 1 Antibody (PA5-17308) in ICC/IF

Immunofluorescent analysis of Alpha-Actinin in NIH/3T3 cells using an Alpha-Actinin polyclonal antibody (Product # PA5-17308) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.

alpha Actinin 1 Antibody in Western Blot (WB)

alpha Actinin 1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

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Product Details

PA5-17308

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:25

View 5 publications 5 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to carboxy-terminal residues of human a-actinin-1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10986146

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

Alpha Actinin 1 belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer.

Journal of translational medicine

Wang C,Xie B,Yin S,Cao J,Huang J,Jin L,Du G,Zhai X,Zhang R,Li S,Cao T,Yu H,Fan X,Yang Z,Peng J,Xiao J,Lian L

PA5-17308 was used in Immunocytochemistry-immunofluorescence to propose ACTN2 as a druggable molecular vulnerability that may provide potential therapeutic benefit against BMM of GC.

Mon Jun 19 00:00:00 EDT 2023

Saffron: A Multitask Neuroprotective Agent for Retinal Degenerative Diseases.

Antioxidants (Basel, Switzerland)

Di Marco S,Carnicelli V,Franceschini N,Di Paolo M,Piccardi M,Bisti S,Falsini B

PA5-17308 was used in Western Blotting to show a way of action of saffron treatment confirming the complex nature of neuroprotective activities of its chemical components.

Wed Jul 17 00:00:00 EDT 2019

Increased free Zn²⁺ correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn²⁺ -transporters in heart failure.

Journal of cellular and molecular medicine

Olgar Y,Durak A,Tuncay E,Bitirim CV,Ozcinar E,Inan MB,Tokcaer-Keskin Z,Akcali KC,Akar AR,Turan B

Published figure using alpha Actinin polyclonal antibody (Product # PA5-17308) in Western Blot

Thu Mar 01 00:00:00 EST 2018

Generation of two human isogenic iPSC lines from fetal dermal fibroblasts.

Stem cell research

Tandon R,Brändl B,Baryshnikova N,Landshammer A,Steenpaß L,Keminer O,Pless O,Müller FJ

PA5-17308 was used in Immunocytochemistry-immunoflourescence to show that both new cell lines offer the opportunity to study basic stem cell biology and model human disease.

Sat Dec 01 00:00:00 EST 2018

Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia.

Nature communications

Latham SL,Ehmke N,Reinke PYA,Taft MH,Eicke D,Reindl T,Stenzel W,Lyons MJ,Friez MJ,Lee JA,Hecker R,Frühwald MC,Becker K,Neuhann TM,Horn D,Schrock E,Niehaus I,Sarnow K,Grützmann K,Gawehn L,Klink B,Rump A,Chaponnier C,Figueiredo C,Knöfler R,Manstein DJ,Di Donato N

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

Variants in exons 5 and 6 of ACTB cause syndromic thrombocytopenia.

Nature communications

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Fri Oct 12 00:00:00 EDT 2018

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.

International journal of molecular medicine

Park S,Choi Y,Jung N,Yu Y,Ryu KH,Kim HS,Jo I,Choi BO,Jung SC

Published figure using alpha Actinin polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Sun May 01 00:00:00 EDT 2016

Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration.

International journal of molecular medicine

Park S,Choi Y,Jung N,Yu Y,Ryu KH,Kim HS,Jo I,Choi BO,Jung SC

Published figure using alpha Actinin polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Sun May 01 00:00:00 EDT 2016

Use of Adeno-Associated Virus to Enrich Cardiomyocytes Derived from Human Stem Cells.

Human gene therapy. Clinical development

Guan X,Wang Z,Czerniecki S,Mack D,François V,Blouin V,Moullier P,Childers MK

Published figure using alpha Actinin 1 polyclonal antibody (Product # PA5-17308) in Immunofluorescence

Tue Sep 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: actinin 1 smooth muscle; actinin, alpha 1; alpha actinin 1a; Alpha-actinin cytoskeletal isoform; Alpha-actinin-1; F-actin cross-linking protein; FLJ40884; FLJ54432; non-muscle alpha-actinin 1; Non-muscle alpha-actinin-1

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Gene Aliases: 3110023F10Rik; ACTN1; Actn1a; BDPLT15; I79_004057

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UniProt ID: (Human) P12814, (Rat) Q9Z1P2, (Mouse) Q7TPR4

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Entrez Gene ID: (Human) 87, (Rat) 81634, (Mouse) 109711, (Chinese hamster) 100755831

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Function(s)

double-stranded RNA binding integrin binding calcium ion binding protein binding vinculin binding ligand-dependent nuclear receptor transcription coactivator activity protein homodimerization activity ion channel binding actin filament binding protein domain specific binding actin binding metal ion binding

Process(es)

platelet degranulation actin filament organization platelet activation platelet formation platelet morphogenesis regulation of apoptotic process focal adhesion assembly actin filament bundle assembly negative regulation of cellular component movement actin filament network formation actin crosslink formation platelet aggregation regulation of nucleic acid-templated transcription cortical cytoskeleton organization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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alpha Actinin 1 Polyclonal Antibody

alpha Actinin 1 Antibody (PA5-17308) in ICC/IF

Product Details

PA5-17308

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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