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Invitrogen

Amyloid Fibrils Polyclonal Antibody

2 References

View all (2) Amyloid Fibrils antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

Application

Amyloid Fibrils Antibody in Western Blot (WB)

FIGURE: 1 / 1

Amyloid Fibrils Antibody (PA5-77843) in WB

Western blot analysis of Amyloid Fibrils in human Abeta42 fibrils and prefibrillar oligomers. The sample was incubated with Amyloid Fibrils polyclonal antibody (Product # PA5-77843) using a dilution of 1:1000.

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Product Details

PA5-77843

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 2 publications 2 publications

ELISA (ELISA)

Assay-Dependent

Immunoprecipitation (IP)

Assay-Dependent

Dot blot (DB)

1:1,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fibrils prepared from human amyloid beta 42 peptide

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735453

Product Specific Information

Specificity: recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrilllar oligomers or natively folded proteins. Expected to detect Mouse and Rat based on species homology.

A 1:1,000 dilution of PA5-77843 was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.

Target Information

Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-beta peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of alpha-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Feedback Loop between TGF-β1 and ATG5 Mediated by miR-122-5p Regulates Fibrosis and EMT in Human Trabecular Meshwork Cells.

Current issues in molecular biology

Chakraborthy M,Rao A

PA5-77843 was used in Immunocytochemistry-immunofluorescence to show that TGF-β1 induces autophagy in primary HTM cells and a positive feedback loop exists between TGF-β1 and ATG5 that regulated TGF downstream effects mainly mediated by Smad3 signaling with miR-122-5p also playing a role.

Mon Mar 13 00:00:00 UTC 2023

Primary Human Trabecular Meshwork Model for Pseudoexfoliation.

Cells

Chakraborty M,Sahay P,Rao A

PA5-77843 was used in Immunocytochemistry-immunofluorescence to create a workable in vitro model of pseudoexfoliation syndrome (PXF) using primary human TM (HTM) cell lines simulating human disease.

Tue Dec 07 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: A11; ABPP; AD; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; APP; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CVAP; Fibrils; OC; peptidase nexin-II; PN-II; PreA4; Protease nexin-II; testicular tissue protein Li 2

Gene Aliases: A4; AAA; ABETA; ABPP; AD1; APP; APPI; CTFgamma; CVAP; PN-II; PN2

UniProt ID: (Human) P05067

Entrez Gene ID: (Human) 351

Function(s)

DNA binding serine-type endopeptidase inhibitor activity receptor binding protein binding heparin binding peptidase activator activity enzyme binding identical protein binding transition metal ion binding PTB domain binding growth factor receptor binding protease inhibitor

Process(es)

response to yeast suckling behavior platelet degranulation mRNA polyadenylation regulation of translation protein phosphorylation cellular copper ion homeostasis endocytosis response to oxidative stress cell adhesion regulation of epidermal growth factor-activated receptor activity Notch signaling pathway axonogenesis mating behavior locomotory behavior axo-dendritic transport cholesterol metabolic process adult locomotory behavior visual learning cellular process response to lead ion negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle axon midline choice point recognition neuron remodeling dendrite development antibacterial humoral response antifungal humoral response extracellular matrix organization forebrain development neuron projection development ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth regulation of protein binding cellular protein metabolic process innate immune response negative regulation of neuron differentiation positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter collateral sprouting in absence of injury regulation of synapse structure or activity defense response to Gram-negative bacterium defense response to Gram-positive bacterium neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis cellular response to cAMP cellular response to norepinephrine stimulus amyloid fibril formation cellular response to nerve growth factor stimulus

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Amyloid Fibrils Polyclonal Antibody

Amyloid Fibrils Antibody (PA5-77843) in WB

Product Details

PA5-77843

Western Blot (WB)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Dot blot (DB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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