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Invitrogen
Ankyrin B Monoclonal Antibody (2.2)
Application
FIGURE: 1 / 6
Ankyrin B Antibody (33-3700) in ICC/IF
Immunofluorescent analysis of Ankyrin B was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Ankyrin B Mouse monoclonal Antibody (Product # 33-3700) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperat... View More
Product Details
33-3700
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Fruit fly,
Hamster,
Insect,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2.2
Immunogen
Synthetic peptide derived from the human ankyrin B protein
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2533115
Target Information
This gene encodes a member of the ankyrin family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton. Ankyrins play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. The protein encoded by this gene is required for targeting and stability of Na/Ca exchanger 1 in cardiomyocytes. Mutations in this gene cause long QT syndrome 4. Multiple transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ANK-2; ank2; ankyrin 2, brain; ankyrin 3, epithelial; ankyrin B; ankyrin, brain; Ankyrin-2; ankyrin-2, nonerythrocytic; Ankyrin-B; Brain ankyrin; Non-erythroid ankyrin
Gene Aliases: AI835472; ANK-2; ANK2; Ank3; ANKB; AW491075; brank-2; LQT4
UniProt ID: (Human) Q01484, (Mouse) Q8C8R3
Entrez Gene ID: (Human) 287, (Mouse) 109676, (Rat) 362036
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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