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Invitrogen

Aquaporin 4 Polyclonal Antibody

Advanced Verification

2 Published Figures

6 References

View all (36) Aquaporin 4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Application

Aquaporin 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

Aquaporin 4 Antibody (PA5-53234) in IHC (P)

Immunohistochemical analysis of Aquaporin 4 in human stomach using Aquaporin 4 Polyclonal Antibody (Product # PA5-53234) shows strong membranous positivity in glandular cells.

Aquaporin 4 Antibody in Immunohistochemistry (IHC)

Aquaporin 4 Antibody in Western Blot (WB)

Aquaporin 4 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

PA5-53234

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:2,500-1:5,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Aquaporin 4. Recombinant protein control fragment (Product #RP-91109).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.30 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2638104

Product Specific Information

Immunogen sequence: CPDVEFKRRF KEAFSKAAQQ TKGSYMEVED NRSQVETDDL ILKPGVVHVI DVDRGEEKKG KDQSGEVLSS V

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

Target Information

In skeletal muscle, AQP4 (aquaporin 4 also known as mercurial insensitive water channel), localizes to the sarcolemma of fast-twitch muscle fibers. Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Multisensory gamma stimulation promotes glymphatic clearance of amyloid.

Nature

Murdock MH,Yang CY,Sun N,Pao PC,Blanco-Duque C,Kahn MC,Kim T,Lavoie NS,Victor MB,Islam MR,Galiana F,Leary N,Wang S,Bubnys A,Ma E,Akay LA,Sneve M,Qian Y,Lai C,McCarthy MM,Kopell N,Kellis M,Piatkevich KD,Boyden ES,Tsai LH

Published figure using Aquaporin 4 polyclonal antibody (Product # PA5-53234) in Immunohistochemistry

Fri Mar 01 00:00:00 UTC 2024

Remyelination in neuromyelitis optica spectrum disorder is promoted by edaravone through mTORC1 signaling activation.

Glia

Luo W,Xu H,Xu L,Jiang W,Chen C,Chang Y,Liu C,Tian Z,Qiu X,Xie C,Li X,Chen H,Lai S,Wu L,Cui Y,Tang C,Qiu W

PA5-53234 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to reveal that edaravone promotes OPC maturation and remyelination/lesion repair in NMOSD patients by activating mTORC1 signaling.

Wed Feb 01 00:00:00 UTC 2023

Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.

Frontiers in immunology

Verma SD,Passerat de la Chapelle E,Malkani S,Juran CM,Boyko V,Costes SV,Cekanaviciute E

Published figure using Aquaporin 4 polyclonal antibody (Product # PA5-53234) in Immunocytochemistry

Tue Oct 25 00:00:00 UTC 2022

Astrocytes regulate vascular endothelial responses to simulated deep space radiation in a human organ-on-a-chip model.

Frontiers in immunology

Verma SD,Passerat de la Chapelle E,Malkani S,Juran CM,Boyko V,Costes SV,Cekanaviciute E

Published figure using Aquaporin 4 polyclonal antibody (Product # PA5-53234) in Immunocytochemistry

Tue Oct 25 00:00:00 UTC 2022

A Microfluidic Model of AQP4 Polarization Dynamics and Fluid Transport in the Healthy and Inflamed Human Brain: The First Step Towards Glymphatics-on-a-Chip.

Advanced biology

Soden PA,Henderson AR,Lee E

PA5-53234 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to observe AQP4 depolarization following LPS treatment, suggesting that LPS-induced drainage impairments on-chip may be driven in part by changes in AQP4-dependent fluid dynamics.

Thu Dec 01 00:00:00 UTC 2022

Engineered human blood-brain barrier microfluidic model for vascular permeability analyses.

Nature protocols

Hajal C,Offeddu GS,Shin Y,Zhang S,Morozova O,Hickman D,Knutson CG,Kamm RD

PA5-53234 was used in Immunohistochemistry (PFA fixed) to show that these properties, coupled with a brain endothelial expression profile and the capability to easily test several repeats with low reagent consumption, make this BBB model highly suitable for widespread use in academic and industrial laboratories.

Sat Jan 01 00:00:00 UTC 2022

Bioinformatics

Protein Aliases: AQP-4; aquaporin type4; Aquaporin-4; Aquaporin4; HMIWC2; Mercurial-insensitive water channel; MGC22454; MIWC; WCH4

Gene Aliases: AQP4; MIWC; WCH4

UniProt ID: (Human) P55087, (Mouse) P55088

Entrez Gene ID: (Human) 361, (Mouse) 11829

Function(s)

water transmembrane transporter activity water channel activity glycerol channel activity transporter activity porin activity transporter

Process(es)

renal water homeostasis transport water transport cellular water homeostasis glycerol transport ion transmembrane transport multicellular organismal water homeostasis cellular response to interferon-gamma sensory perception of sound regulation of vascular endothelial growth factor production carbon dioxide transport water homeostasis negative regulation of interleukin-1 beta production negative regulation of interleukin-6 production carbohydrate transmembrane transport protein homooligomerization transmembrane transport negative regulation of cell adhesion molecule production renal water absorption cellular response to glucose stimulus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Aquaporin 4 Polyclonal Antibody

Aquaporin 4 Antibody (PA5-53234) in IHC (P)

Product Details

PA5-53234

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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Aquaporin 4 Polyclonal Antibody

Aquaporin 4 Antibody (PA5-53234) in IHC (P)

Product Details

PA5-53234

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

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