Invitrogen

Aromatase Polyclonal Antibody

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Antibody Testing Data (1)
Published Figures (4)

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Aromatase Antibody (PA1-21398) in WB

Western Blot analysis of human brain lysate using Aromatase Polyclonal Antibody (Product # PA1-21398).

Aromatase Antibody in Immunohistochemistry (IHC)

Aromatase Antibody in Western Blot, Immunohistochemistry (WB, IHC)

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Product Details

PA1-21398

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-4 µg/mL

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

10-20 µg/mL

Product Specifications

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat

Published species

Bovine, Human, Mouse, Pig, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide surrounding amino acid 385 of human Aromatase

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 30% glycerol, 5mM EDTA, 0.5% BSA

Contains

0.03% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2088676

Product Specific Information

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development and Characterization of Inducible Astrocyte-Specific Aromatase Knockout Mice.

Biology

Wang J,Pratap UP,Lu Y,Sareddy GR,Tekmal RR,Vadlamudi RK,Brann DW

PA1-21398 was used in Western Blotting to develop a tamoxifen (TMX)-inducible astrocyte-specific aromatase knockout mouse line (GFAP-ARO-iKO mice) to specifically deplete the E2 synthesis enzymes and aromatase in astrocytes after their development in adult mice.

Wed Apr 19 00:00:00 EDT 2023

KISS1R and ANKRD31 Cooperate to Enhance Leydig Cell Gene Expression via the Cytoskeletal-Nucleoskeletal Pathway.

Frontiers in cell and developmental biology

Ricci G,Guillou F,Catizone A,Mele VG,Moggio M,Chioccarelli T,Diano N,Meccariello R,Pierantoni R,Fasano S,Cobellis G,Chianese R,Manfrevola F

PA1-21398 was used in Western Blotting to highlight a novel role for the Kisspeptin-10 in the induction of the cytoskeletal-nucleoskeletal route, downstream a physical interaction between KISS1 receptor and ANKRD31, with gene expression activation as final effect, in Leydig cells.

Sat Jul 16 00:00:00 EDT 2022

High Doses of D-Chiro-Inositol Alone Induce a PCO-Like Syndrome and Other Alterations in Mouse Ovaries.

International journal of molecular sciences

Bevilacqua A,Dragotto J,Lucarelli M,Di Emidio G,Monastra G,Tatone C

PA1-21398 was used in Western Blot to analyze effects of higher doses of DCIns, 5, 10 and 20 mg/day, administered to young adult female mice for 21 days, on ovarian histology, serum testosterone levels and expression of the ovarian enzyme aromatase.

Wed May 26 00:00:00 EDT 2021

High Doses of D-Chiro-Inositol Alone Induce a PCO-Like Syndrome and Other Alterations in Mouse Ovaries.

International journal of molecular sciences

Bevilacqua A,Dragotto J,Lucarelli M,Di Emidio G,Monastra G,Tatone C

Wed May 26 00:00:00 EDT 2021

The role of vaspin in porcine corpus luteum.

The Journal of endocrinology

Kurowska P,Mlyczyńska E,Dawid M,Grzesiak M,Dupont J,Rak A

PA1-21398 was used in Western Blot to find that vaspin/GRP78 expression depends on the luteal phase stage and vaspin affects luteal cells endocrinology, indicating that vaspin is a new regulator of luteal cells steroidogenesis and CL formation.

Tue Dec 01 00:00:00 EST 2020

Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†.

Biology of reproduction

Kurowska P,Mlyczyńska E,Dawid M,Dupont J,Rak A

Published figure using Aromatase polyclonal antibody (Product # PA1-21398) in Western Blot

Tue May 26 00:00:00 EDT 2020

INSIGHTS ON AROMATASE IMMUNOHISTOCHEMISTRY: VARIATIONS BETWEEN INTRINSIC MOLECULAR SUBTYPES OF BREAST CANCERS.

Acta endocrinologica (Bucharest, Romania : 2005)

Armasu I,Preda C,Ianole V,Mocanu V,Hristov I,Andriescu EC,Cretu-Silivestru I,Vasiliu I,Dascalu CG,Lupascu CD,Crumpei I,Serban DN,Serban IL,Ciobanu Apostol DG

PA1-21398 was used in Immunohistochemistry to describe aromatase expression in the major tissue compartments of breast malignant tumors.

Fri Apr 15 00:00:00 EDT 2022

Developmental programming: Changes in mediators of insulin sensitivity in prenatal bisphenol A-treated female sheep.

Reproductive toxicology (Elmsford, N.Y.)

Puttabyatappa M,Martin JD,Andriessen V,Stevenson M,Zeng L,Pennathur S,Padmanabhan V

PA1-21398 was used in Western Blotting to determine if changes in insulin sensitivity mediators (increase in inflammation, oxidative stress, and lipotoxicity and/or decrease in adiponectin) and the intracrine steroidal milieu contributes to these metabolic perturbations.

Mon Apr 01 00:00:00 EDT 2019

Tanshinone IIA attenuates estradiol-induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary.

Experimental and therapeutic medicine

Jin J,Hu QY,Xu WW,Zhu WJ,Liu B,Liu J,Wang W,Zhou HF

PA1-21398 was used in Western Blotting to replicate PCOS via the neonatal treatment of estradiol in mice.

Wed May 01 00:00:00 EDT 2019

Increased expression of aromatase cytochrome P450 enzyme is associated with prolactinoma invasiveness in post-menopausal women.

The Journal of international medical research

Su YX,Du GL,Shen HL,Wang W,Bao JL,Aierken A,Wang BW,Jiang S,Zhu J,Gao XM

Published figure using Aromatase polyclonal antibody (Product # PA1-21398) in Immunohistochemistry

Mon Jul 01 00:00:00 EDT 2019

Developmental Programming: Impact of Prenatal Testosterone Excess on Steroidal Machinery and Cell Differentiation Markers in Visceral Adipocytes of Female Sheep.

Reproductive sciences (Thousand Oaks, Calif.)

Puttabyatappa M,Lu C,Martin JD,Chazenbalk G,Dumesic D,Padmanabhan V

PA1-21398 was used in Western Blotting to show that increased commitment of AT-MSCs to adipocyte lineage and decreased differentiation to adipocytes may underlie the small adipocyte phenotype of prenatal T-treated females.

Sun Jul 01 00:00:00 EDT 2018

Aromatase enzyme expression in acromegaly and its possible relationship with disease prognosis.

Endocrine

Selek A,Cetinarslan B,Gurbuz Y,Tarkun I,Canturk Z,Cabuk B

PA1-21398 was used in immunohistochemistry to assess the relationship between aromatase expression and the clinical and prognostic features growth hormone secreting adenomas, prolactinomas, and normal pituitary tissues

Fri May 01 00:00:00 EDT 2015

Developmental programming: prenatal steroid excess disrupts key members of intraovarian steroidogenic pathway in sheep.

Endocrinology

Padmanabhan V,Salvetti NR,Matiller V,Ortega HH

Published figure using Aromatase polyclonal antibody (Product # PA1-21398) in Immunohistochemistry

Mon Sep 01 00:00:00 EDT 2014

Developmental programming: prenatal steroid excess disrupts key members of intraovarian steroidogenic pathway in sheep.

Endocrinology

Padmanabhan V,Salvetti NR,Matiller V,Ortega HH

Published figure using Aromatase polyclonal antibody (Product # PA1-21398) in Immunohistochemistry

Mon Sep 01 00:00:00 EDT 2014

Pathogen-associated molecular patterns initiate inflammation and perturb the endocrine function of bovine granulosa cells from ovarian dominant follicles via TLR2 and TLR4 pathways.

Endocrinology

Price JC,Bromfield JJ,Sheldon IM

PA1-21398 was used in western blot to study the role of TLR2 and TLR4 in the initiation of inflammation and disturbance of bovine granulosa cell endocrine function by bacterial PAMPs

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Aromatase; Aromatase 1; aromatase cytochrome P450; aromatase cytochrome P450 19A1; Cyp19; CYPXIX; CYPXIXA1; cytochrome P-450 aromatase; cytochrome P-450 aromatase (alt.); Cytochrome P-450AROM; Cytochrome P450 17-alpha; Cytochrome P450 19 type I; Cytochrome P450 19A1; cytochrome P450 aromatase; cytochrome P450 family 19 subfamily a; Cytochrome P450, 19, aromatase; cytochrome P450, family 19, subfamily A, polypeptide 1; cytochrome P450, family XIX (conversion of androgen to estrogen), aromatase; cytochrome P450, family19, subfamily A, polypeptide 1; cytochrome P450, subfamily 19; cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; MGC104309; microsomal monooxygenase; P-450AROM; type III cytochrome p450 aromatase

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Gene Aliases: Ar; ArKO; ARO; ARO1; Arom; Aromatase; CPV1; CYAR; CYP19; Cyp19a; CYP19A1; CYP19P1; CYPXIX; Int-5; Int5; P-450AROM; P450AROM

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UniProt ID: (Human) P11511, (Rabbit) Q29605, (Bovine) P46194, (Pig) Q29624, (Horse) O46512, (Mouse) P28649

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Entrez Gene ID: (Human) 1588, (Chicken) 414854, (Rabbit) 100328545, (Bovine) 281740, (Pig) 403331, (Horse) 100009712, (Mouse) 13075, (Rat) 25147

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Function(s)

iron ion binding steroid hydroxylase activity electron carrier activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen oxygen binding heme binding aromatase activity monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding oxygenase

Process(es)

negative regulation of chronic inflammatory response steroid biosynthetic process estrogen biosynthetic process androgen metabolic process negative regulation of macrophage chemotaxis sterol metabolic process oxidation-reduction process prostate gland growth female gonad development response to estradiol C21-steroid hormone metabolic process negative regulation of bone resorption regulation of synapse structure or activity luteinization bile acid metabolic process male gonad development response to herbicide response to metal ion response to cesium ion response to ionizing radiation response to organic cyclic compound biphenyl metabolic process dibenzo-p-dioxin metabolic process phthalate metabolic process hippocampus development hypothalamus development melatonin metabolic process adrenal gland development granulocyte differentiation response to testosterone progesterone metabolic process response to drug response to morphine response to estrogen cellular response to fibroblast growth factor stimulus bone development cellular response to glucose stimulus cellular response to gonadotropin stimulus cellular response to follicle-stimulating hormone stimulus cellular response to hydrostatic pressure

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Aromatase Polyclonal Antibody

Aromatase Antibody (PA1-21398) in WB

Product Details

PA1-21398

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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