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            • Primary Antibodies ›
            • GOT1 Antibodies

            Rockland

            Aspartate Transaminase Polyclonal Antibody

            View all (32) GOT1 antibodies
            Datasheet
            Protocols
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            Datasheet
            Protocols
            Questions & Answers

            Cite Aspartate Transaminase Polyclonal Antibody

            Product Details

            200-601-144-0100

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            1:1,000
            -

            ELISA (ELISA)

            1:5,000-1:25,000
            -

            Immunoprecipitation (IP)

            1:500-1:3,000
            -
            Product Specifications

            Species Reactivity

            Pig

            Host/Isotype

            Sheep / IgG

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            Anti-Aspartate Transaminase antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart.
            View immunogen

            Conjugate

            Unconjugated

            Form

            Lyophilized

            Concentration

            1 mg/mL

            Purification

            Ion-exchange chromatography

            Storage buffer

            0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

            Contains

            0.01% sodium azide

            Storage conditions

            Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            Product Specific Information

            Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

            Anti-Aspartate Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Aspartate Transaminase (AST) [Pig Heart]. Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined.

            Target Information

            GOT1 is a glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

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            Cite this product

            Bioinformatics

            Protein Aliases: Aspartate aminotransferase, cytoplasmic; cAspAT; cCAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; Glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1); Transaminase A

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            Gene Aliases: CASPAT; cCAT; GOT1

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            UniProt ID: (Pig) P00503

            View more View less

            Entrez Gene ID: (Pig) 396967

            View more View less

            Function(s)

            L-aspartate:2-oxoglutarate aminotransferase activity phosphatidylserine decarboxylase activity pyridoxal phosphate binding L-cysteine:2-oxoglutarate aminotransferase activity transaminase

            Process(es)

            2-oxoglutarate metabolic process oxaloacetate metabolic process glycerol biosynthetic process aspartate metabolic process aspartate catabolic process glutamate metabolic process Notch signaling pathway glutamate catabolic process to aspartate glutamate catabolic process to 2-oxoglutarate cellular response to insulin stimulus response to glucocorticoid fatty acid homeostasis
            It has to be done as per old AB suggested Products section.
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            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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