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Bethyl Laboratories
Astrin/MAP126 Polyclonal Antibody
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Application
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Astrin/MAP126 Antibody (A301-512A) in WB
Detection of human Astrin/MAP126 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Astrin/MAP126 antibody (Product # A301-512A) used for WB at 0.04 µg/mL (A) and 0.1 µg/mL (B) and used for IP at 3 µg/mg lysate. Astrin/MAP126 was also immunoprecipitated by rabbit anti-Astrin/MAP126 antibodies A301-511A and BL5929, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds... View More
Product Details
A301-512A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 875 and 925 of human astrin (mitotic spindle associated protein p126).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1,193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Astrin; Deepest; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; Sperm-associated antigen 5
Gene Aliases: DEEPEST; hMAP126; MAP126; SPAG5
UniProt ID: (Human) Q96R06
Entrez Gene ID: (Human) 10615
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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