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Invitrogen
B7-H7 Monoclonal Antibody (MA57YW), APC, eBioscience™
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Application
FIGURE: 1 / 2
B7-H7 Antibody (17-6537-42) in Flow
HDLM-2 cells were stained with Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (blue) or B7-H7 Monoclonal Antibody, APC (purple). Total viable cells, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18), were used for analysis.
Product Details
17-6537-42
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MA57YW
Immunogen
B7-H7 recombinant protein
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784652
Product Specific Information
Description: This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells.
Applications Reported: This MA57YW antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MA57YW antibody has been pre-diluted and tested by flow cytometric analysis of the HDLM2 cell line. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
Target Information
B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), whereas other members of the B7 family generally have only two Ig domains (IgV-IgC). B7-H7 is somewhat more closely related to B7-H3 and B7-H4 and shares 10% to 18% amino acid identity and 23% to 33% similarity to B7 family members. B7-H7 protein is widely expressed in epithelium cells of the gallbladder, breast, kidney, colon, small intestines and placenta. In the immune system, HHLA2 protein is not constitutively expressed on human monocytes/macrophages. B7-H7 is widely expressed in cancers, especially in triple negative breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HERV-H LTR-associating protein 2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
Gene Aliases: B7-H5; B7-H7; B7H7; B7y; HHLA2
UniProt ID: (Human) Q9UM44
Entrez Gene ID: (Human) 11148
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