Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Primary Antibodies
  • Search All
  • Popular Product Areas
  • TaqMan Assays
  • Primary Antibodies
  • Secondary Antibodies
  • Isotype Controls
  • Proteins & Peptides
  • ELISA Kits
  • All Documents & Support
  • Certificates
  • SDS
  • Manuals & Protocols
  • Product FAQs
    Search button Close
            Target of Interest
            Application
            Target Species
            • Order Status
            • Quick Order
            • Sign in
              Sign in
              Don't have an account ? Create Account
              • Account
              • Check Order Status
              • Aspire Member Program
              • Connect: Lab, Data, Apps
              • Custom Products & Projects
              • Services Central

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Primary Antibodies ›
            • BAFF (BLyS) Antibodies
            The PeproTech website has now moved to Thermofisher.com. All orders for PeproTech products must be placed on thermofisher.com or offline via phone, fax, email, or distributor partner. For any questions, please contact PeproTech.CustomerService@thermofisher.com

            Invitrogen

            BAFF Polyclonal Antibody, Biotin, PeproTech®

            View all (35) BAFF (BLyS) antibodies
            Datasheet
            Protocols
            Questions & Answers
            Catalog # 500-P163BT-25UG
            Web quote
            You can generate a web quote (PDF) by signing in to your account and adding products to your cart. View cart and click the 'Generate a web quote' button. With your web quote document, you can get approval for your order and complete your order online.
             
            Price (KRW)
            303,000
            Online offer
            Ends: 30-Sep-2025
            336,000
            Save 33,000 (10%)
             
            Check your price
            Request bulk or custom format
            Join Aspire™ for free antibodies
            Datasheet
            Protocols
            Questions & Answers

            Cite BAFF Polyclonal Antibody, Biotin, PeproTech®

            • Antibody Testing Data (3)
            Application
            BAFF Antibody in Western Blot (WB)
            Group 53 Created with Sketch.

            FIGURE: 1 / 3

            BAFF Antibody (500-P163BT-25UG) in WB

            Western Blot: To detect Human BAFF by Western Blot analysis BAFF Polyclonal Antibody, Biotin (Product # 500-P163BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
            View Product

            BAFF Antibody in Western Blot (WB)
            BAFF Antibody in Western Blot (WB)
            BAFF Antibody in ELISA (ELISA)

            Product Details

            500-P163BT-25UG

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            0.1-0.2 µg/mL
            -

            ELISA (ELISA)

            0.25-1.0 µg/mL
            -
            Product Specifications

            Species Reactivity

            Human

            Host/Isotype

            Rabbit

            Class

            Polyclonal

            Type

            Antibody

            Immunogen

            E.coli-derived Recombinant Human BAFF
            View immunogen

            Conjugate

            Biotin

            Form

            Lyophilized

            Concentration

            0.1-1.0 mg/mL

            Purification

            Antigen affinity chromatography

            Storage buffer

            PBS

            Contains

            no preservative

            Storage conditions

            -20°C

            Shipping conditions

            Ambient

            RRID

            AB_2929573

            Product Specific Information

            AA Sequence of recombinant protein: AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL.

            Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BAFF. Anti-Human BAFF-specific antibody was purified by affinity chromatography and then biotinylated.

            Sandwich ELISA: To detect Human BAFF by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BAFF (500-P163) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human BAFF.

            Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human BAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

            500-P163BT-1MG will be provided as 2 x 500 µg

            Target Information

            BAFF is a membrane bound cytokine expressed by B cell lineage cells. The soluble form of the cytokine binds to the receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. BAFF is a potent B cell activator, and important for the proliferation and differentiation of B cells. BAFF is also expressed by T lymphocytes and has been shown to promote T cell activation and survival. Isoforms representing alternatively spliced, and glycosylated variants have been identified.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            References (0)

            Have you cited this product in a publication?
            Let us know so we can reference it here.
            Cite this product

            Bioinformatics

            Protein Aliases: ApoL related ligand TALL-1; B lymphocyte stimulator; B-cell-activating factor; B-lymphocyte stimulator; BAFF; BLyS; CD257; delta BAFF; Delta4 BAFF; Dendritic cell-derived TNF-like molecule; TALL-1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNF- and APOL-related leukocyte expressed ligand 1; tumor necrosis factor (ligand) superfamily member 13b; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor-like protein ZTNF4

            View more View less

            Gene Aliases: BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF13B; TNFSF20; TNLG7A; UNQ401/PRO738; ZTNF4

            View more View less

            UniProt ID: (Human) Q9Y275

            View more View less

            Entrez Gene ID: (Human) 10673

            View more View less

            Function(s)

            receptor binding cytokine activity tumor necrosis factor receptor binding protein binding

            Process(es)

            immune response signal transduction cell proliferation positive regulation of cell proliferation positive regulation of B cell proliferation tumor necrosis factor-mediated signaling pathway
            It has to be done as per old AB suggested Products section.
            guarantee_icon

            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
            help_icon

            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

            Contact tech support
            Ordering Plus Icon Minus Icon
            • Order Status
            • Order Help
            • Quick Order
            • Supply Center
            • eProcurement
            Support Plus Icon Minus Icon
            • Help and Support
            • Contact Us
            • Technical Support Centers
            • Documents and Certificates
            • Report a Site Issue
            Resources Plus Icon Minus Icon
            • Learning Centers
            • Promotions
            • Events and Webinars
            • Social Media
            About Thermo Fisher Plus Icon Minus Icon
            • About Us
            • Careers
            • Investors
            • News
            • Social Responsibility
            • Trademarks
            • Consumer Health Data Privacy Policy
            Our Portfolio Plus Icon Minus Icon
            • Thermo Scientific
            • Applied Biosystems
            • Invitrogen
            • Gibco
            • Ion Torrent
            • Fisher Scientific
            • Unity Lab Services
            • Patheon
            • PPD
            • Terms & Conditions
            • Privacy Information Center
            • Price & Freight Policy
            • Cookie Preferences

              Your choices regarding cookies on this site

              We and our affiliates and vendors use cookies and similar technologies to operate our sites, recognize visitors to our sites, provide secure log-in, collect statistics to optimize site functionality, and deliver content tailored to your interests. Click Accept All to accept all cookies and go directly to the site, click Reject All to reject all but cookies strictly necessary to the functioning of this site (required cookies), or click on Manage Settings to see detailed descriptions of the types of cookies and choose whether to accept certain cookies while on the site.
            © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
            united states flag icon
            United States

            Your items have has been added!


            Host server : magellan-search-green-584499df9d-8sqwf:80/100.66.74.130:80.
            git-commit: 256cec658627a60c8e1196038bf5905a13cd45ba
            git-url: https://github.com/thermofisher/magellan-search
            git-branch: release/2.33.0-2025.07.30-1.0