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Invitrogen
BAP31 Monoclonal Antibody (CC-1)
Application
FIGURE: 1 / 20
BAP31 Antibody (MA3-002) in ICC/IF
Immunofluorescence analysis of BAP31 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BAP31 Monoclonal Antibody (Product # MA3-002) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at a dilution of 1:200... View More
Product Details
MA3-002
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
CC-1
Immunogen
Amino acid residues 230-246 of BAP31 protein.
Conjugate
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325095
Product Specific Information
MA3-002 detects BAP31 from human, non-human primate, bovine, mouse and hamster samples.
MA3-002 has been successfully used in Western blot, ELISA, immunoprecipitation, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31.
The MA3-002 antigen is solubilized protein from human neuroendocrine cell line IMR-32 corresponding to the C-terminal region residues 230-246 of BAP31 protein. This sequence is conserved in non-human primate, bovine and hamster species.
Target Information
BAP31 (B-Cell Receptor Associated Protein, 31-kDa) is a multiple membrane spanning protein of the endoplasmic reticulum. It has been shown to form both homo- and hetero-oligomers with the closely related BAP29, and is a potential apoptotic regulator as a predicted BCL-2/BCL-XL-associated protein. In the C-terminal domain of BAP31, there are two identical recognition sites for initiator Caspases 1 and 8 of the programmed death cascade. It is hypothesized that BAP31 plays a role in the transport of selected proteins to the Golgi apparatus. BAP31 has also been shown to control expression of CFTR (cystic fibrosis transmembrane conductance regulator). It is believed that interference with the expression or function of BAP31 in epithelial cells may be a way to circumvent the chloride channel defect in cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 6C6 antigen; 6C6-AG tumor-associated antigen; accessory protein BAP31; B-cell receptor-associated protein 31; BCR-associated protein 31; BCR-associated protein Bap31; CDM protein; OTTHUMP00000025978; p28; p28 Bap31; Protein CDM
Gene Aliases: 6C6-AG; BAP31; BCAP31; CDM; DDCH; DXS1357E
UniProt ID: (Human) P51572, (Mouse) Q61335
Entrez Gene ID: (Human) 10134, (Bovine) 533949, (Chinese hamster) 100770274, (Mouse) 27061
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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