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BDNF Antibody (MA5-23665) in Flow
Flow cytometry of BDNF in U‚87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in BDNF monoclonal antibody (Product # MA5-23665) or isotype control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.
Product Details
MA5-23665
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
35909
Immunogen
Sf 21-derived recombinant human BDNF His129-Arg247
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609147
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant human (rh) NT-3, rhNT-4, or rh beta -NGF is observed.
Target Information
BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Abrineurin; BDNF; Brain-derived neurotrophic factor; H-BDNF; neurotrophin
Gene Aliases: ANON2; BDNF; BULN2
UniProt ID: (Human) Q9BYY7
Entrez Gene ID: (Human) 627
cell-cell signaling
nervous system development
axon guidance
synapse assembly
brain-derived neurotrophic factor receptor signaling pathway
positive regulation of brain-derived neurotrophic factor receptor signaling pathway
negative regulation of neuron apoptotic process
collateral sprouting
positive regulation of collateral sprouting
positive regulation of synapse assembly
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