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Application
FIGURE: 1 / 1
BNIP2 Antibody (PA5-92864) in WB
Western blot analysis of BNIP2 in extracts of various cell lines. Samples were incubated with BNIP2 Polyclonal antibody (Product # PA5-92864) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-92864
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 125-280 of human BNIP2 (NP_0043212)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.67 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806523
Product Specific Information
Immunogen sequence: VELKEEWQDE DFPIPLPEDD SIEADILAIT GPEDQPGSLE VNGNKVRKKL MAPDISLTLD PSDGSVLSDD LDESGEIDLD GLDTPSENSN EFEWEDDLPK PKTTEVIRKG SITEYTAAEE KEDGRRWRMF RIGEQDHRVD MKAIEPYKKV ISHGGY; Positive Samples: U-937, SW480, NCI-H460, Mouse heart; Cellular Location: Cytoplasm, perinuclear region
Target Information
BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2; BCL2/adenovirus E1B 19 kDa-interacting protein 2 beta; BCL2/adenovirus E1B 19kDa interacting protein 2; BCL2/adenovirus E1B 19kDa-interacting protein 1, NIP2; BCL2/adenovirus E1B interacting protein 1, NIP2; BNIP 2
Gene Aliases: 5730523P12Rik; BNIP-2; BNIP2; BNIP2beta; NIP2; Nip21; Nip2l
UniProt ID: (Human) Q12982, (Mouse) Q8K4H0
Entrez Gene ID: (Human) 663, (Mouse) 12175
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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