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Invitrogen
BNIP3 Monoclonal Antibody (ANa40)
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Application
FIGURE: 1 / 2
BNIP3 Antibody (MA1-24688) in WB
Western blot was performed using Anti-BNIP3 Monoclonal Antibody (ANa40)(Product # MA1-24688) and a 28kDa band corresponding to BNIP3 was observed across cell lines tested. Membrane enriched extracts (30ug µg lysate) of HeLa (Lane 1), HeLa treated with Cobalt Chloride, 150uM,48Hrs (Lane 2), MCF7 (Lane 3), MCF7 treated with Cobalt Chloride,150uM,48Hrs (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot®... View More
Product Details
MA1-24688
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
ANa40
Immunogen
Recombinant human BNIP3 (amino acids 1-163, BNIP3 delta TM2).
Conjugate
Unconjugated
Form
Liquid
Concentration
2.4 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2066655
Product Specific Information
Positive Control: 293T cells transfected with BNIP3.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3
Gene Aliases: BNIP3; NIP3
UniProt ID: (Human) Q12983
Entrez Gene ID: (Human) 664
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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