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Application
FIGURE: 1 / 1
BOB-1 Antibody (PA5-121026) in WB
Western blot analysis of BOB-1 in various lysates. Samples were incubated with BOB-1 Polyclonal antibody (Product # PA5-121026) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-121026
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human POU2AF1 (NP_006226.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.92 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914598
Product Specific Information
Positive test controls include: HT-29, Mouse spleen, Mouse thymus, Rat spleen. The target is usually found in the following locations: Nucleus.
Immunogen sequence: MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF
Target Information
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-cell-specific coactivator OBF-1; BOB-1; BOB1; OCA-B; OCT-binding factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1
Gene Aliases: Bob-1; BOB.1; BOB1; OBF-1; OBF.1; OBF1; OCA-B; OCAB; POU2AF1
UniProt ID: (Human) Q16633, (Mouse) Q64693
Entrez Gene ID: (Human) 5450, (Mouse) 18985, (Rat) 690528
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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