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BTAF1 Antibody (A301-063A-T) in WB
Detection of human BTAF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody (Product # A301-063A) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. BTAF1 was immunoprecipitated more efficiently by rabbit anti-BTAF1 antibodies A301-064A and A301-065A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 min... View More
Product Details
A301-063A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 75 and 125 of human RNA polymerase II, B-TFIID transcription factor-associated, 170kDa.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP) and TBP-associated factors, or TAFs (e.g., TAF2B), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); Mot1 homolog; TAF(II)170; TAF-172; TATA-binding protein-associated factor 172; TBP-associated factor 172
Gene Aliases: BTAF1; MOT1; TAF(II)170; TAF172; TAFII170
UniProt ID: (Human) O14981
Entrez Gene ID: (Human) 9044
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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