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BTLA Antibody (PA5-120216) in WB
Western blot analysis of BTLA in extracts of various cell lines. Samples were incubated with BTLA Polyclonal antibody (Product # PA5-120216) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120216
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 31-241 of human BTLA (NP_001078826.1).
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913788
Product Specific Information
Positive test controls include: Jurkat, Mouse spleen, Mouse thymus. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.
Immunogen sequence: KESCDVQLYI KRQSEHSILA GDPFELECPV KYCANRPHVT WCKLNGTTCV KLEDRQTSWK EEKNISFFIL HFEPVLPNDN GSYRCSANFQ SNLIESHSTT LYVTGKQNEL SDTAGREINL VDAHLKSEQT EASTRQNSQV LLSETGIYDN DPDLCFRMQE GSEVYSNPCL EENKPGIVYA SLNHSVIGPN SRLARNVKEA PTEYASICVR S
Target Information
BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B and T lymphocyte associated protein; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA_HUMAN; CD272; CD272 antigen; FLJ16065; MGC129743
Gene Aliases: A630002H24; BTLA; BTLA1; CD272
UniProt ID: (Human) Q7Z6A9, (Mouse) Q7TSA3
Entrez Gene ID: (Human) 151888, (Mouse) 208154
adaptive immune response
T cell costimulation
immune system process
immune response-regulating cell surface receptor signaling pathway
cell surface receptor signaling pathway
negative regulation of B cell proliferation
negative regulation of T cell proliferation
negative regulation of alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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