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FIGURE: 1 / 1
Bcl-X Antibody (PA1-46479) in WB
Western blot analysis of Bcl-X in whole cell extract of A431 cell line. Samples were incubated in Bcl-X polyclonal antibody (Product # PA1-46479). The band at ~26 kDa is the specific target band, whereas the band above ~50 kDa position is potentially a homodimer of BCL2L1.
Product Details
PA1-46479
Product Specifications
Species Reactivity
Dog,
Cat,
Human,
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an N-terminal portion of human BCL2L1 (within residues 30-70). [Swiss-Prot# Q07817]
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.1% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Wet ice
RRID
AB_1956299
Product Specific Information
The target sequence has 93% sequence homology with porcine, 87% to mouse and 81% to rat. This antibody is not recommended for ICC/IF applications.
Target Information
The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: anti-apoptotic Bcl-2 gene family member; Apoptosis regulator Bcl-X; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; bcl-2 L1; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BCL2L1; BclxL; BclXS; DKFZp781P2092; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3
Gene Aliases: Bcl-X; BCL-XL; BCL-XL/S; BCL2L; BCL2L1; BCLX; PPP1R52
UniProt ID: (Human) Q07817
Entrez Gene ID: (Human) 598, (Dog) 403618, (Sheep) 443061, (Cat) 493701
cytokinesis
ovarian follicle development
in utero embryonic development
release of cytochrome c from mitochondria
endocytosis
mitotic cell cycle checkpoint
germ cell development
spermatogenesis
cell proliferation
positive regulation of cell proliferation
male gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
fertilization
suppression by virus of host apoptotic process
response to cytokine
growth
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
response to cycloheximide
regulation of mitochondrial membrane permeability
neuron apoptotic process
regulation of mitochondrial membrane potential
cellular process regulating host cell cycle in response to virus
mitochondrion morphogenesis
cellular response to amino acid stimulus
cellular response to alkaloid
cellular response to gamma radiation
apoptotic process in bone marrow
negative regulation of establishment of protein localization to plasma membrane
negative regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway in absence of ligand
hepatocyte apoptotic process
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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