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Invitrogen
beta-5 Tubulin Polyclonal Antibody
Application
FIGURE: 1 / 13
beta-5 Tubulin Antibody (PA5-80198) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Beta III Tubulin in U2OS cells using Beta III Tubulin Polyclonal Antibody (Product # PA5-80198). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. Cy3 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:... View More
Product Details
PA5-80198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin (383-412aa EQFTAMFRRKAFLHWYTGEGMDEMEFTEAE).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2747312
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human SiHa whole cell, human 293T whole cell, human HepG2 whole cell, monkey COS-7 whole cell, chicken heart tissue, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human liver cancer tissue, human testicular germ cell tumor tissue. ICC/IF: U20S cell. Flow: SiHa cell.
Target Information
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tublin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. This antibody does not cause the 10-nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. Ab-3 does not inhibit polymerization or depolymerization of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: beta 5-tubulin; beta Ib tubulin; c(beta)7 tubulin; tbb5; tubulin beta; Tubulin beta 4'; Tubulin beta chain; tubulin beta-1 chain; Tubulin beta-5 chain; Tubulin beta-7 chain; tubulin, beta 2A; tubulin, beta class I; tubulin, beta polypeptide; unnamed protein product
Gene Aliases: AA408537; AI596182; B130022C14Rik; CDCBM6; CSCSC1; M(beta)5; M40; OK/SW-cl.56; TUBB; TUBB1; TUBB5
UniProt ID: (Human) P07437, (Chicken) P09244, (Mouse) P99024, (Rat) P69897
Entrez Gene ID: (Human) 203068, (Chicken) 396254, (Mouse) 22154, (Rat) 29214
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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