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Invitrogen

Brachyury Polyclonal Antibody

2 References

View all (34) Brachyury antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (7)

Application

Brachyury Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

Brachyury Antibody (PA5-46984) in ICC/IF

Immunocytochemistry analysis of Brachyury in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm. Samples were incubated in Brachyury polyclonal antibody (Product # PA5-46984) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was locali... View More

Brachyury Antibody in Immunohistochemistry (Frozen) (IHC (F))

Brachyury Antibody in Flow Cytometry (Flow)

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Product Details

PA5-46984

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

5-15 µg/mL

Immunocytochemistry (ICC/IF)

5-15 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/10^6 cells

ChIP assay (ChIP)

5 µg per million cells

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human Brachyury Ser2-Glu202

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2610378

Product Specific Information

In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-cell analysis of embryoids reveals lineage diversification roadmaps of early human development.

Cell stem cell

Zheng Y,Yan RZ,Sun S,Kobayashi M,Xiang L,Yang R,Goedel A,Kang Y,Xue X,Esfahani SN,Liu Y,Resto Irizarry AM,Wu W,Li Y,Ji W,Niu Y,Chien KR,Li T,Shioda T,Fu J

PA5-46984 was used in Immunohistochemistry to propose stringent criteria for distinguishing between human blastocyst trophectoderm and early amniotic ectoderm cells.

Thu Sep 01 00:00:00 UTC 2022

A microfluidics-based stem cell model of early post-implantation human development.

Nature protocols

Zheng Y,Shao Y,Fu J

PA5-46984 was used in ChIP Assay, Immunocytochemistry, Immunohistochemistry (Frozen), Western Blot to provide details of the design and implementation of a microfluidic device that can be used to model human embryo development.

Fri Jan 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

Gene Aliases: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3

UniProt ID: (Human) O15178, (Mouse) P20293

Entrez Gene ID: (Human) 6862, (Mouse) 20997

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding protein binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development anatomical structure morphogenesis anterior/posterior pattern specification reproductive process notochord development positive regulation of transcription from RNA polymerase II promoter cardiac muscle cell differentiation

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