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Invitrogen

Brachyury Polyclonal Antibody

1 Reference

View all (34) Brachyury antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)

Application

Brachyury Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

Brachyury Antibody (PA5-78422) in IHC (P)

Immunohistochemistry (Paraffin) analysis of Brachyury was performed in paraffin-embedded mouse E105 mouse embryo tissue using Green: TRPC6 Polyclonal Antibody (Product # PA5-28100) at a dilution of 1:250. Red: SOX2, a nucleus marker, stained by SOX2 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.

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Product Details

PA5-78422

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Human Brachyury. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735534

Product Specific Information

Positive Control: mouse testis

Predicted Reactivity: Human (100%), Rat (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reproducible differentiation and characterization of neurons from mouse embryonic stem cells.

MethodsX

Saxena S,Choudhury S,Mohan KN

PA5-78422 was used in Immunocytochemistry-immunoflourescence to describe a 16-day differentiation protocol that consistently induced differentiation of mESCs into neurons.

Thu Oct 22 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

Gene Aliases: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3

UniProt ID: (Human) O15178, (Mouse) P20293

Entrez Gene ID: (Human) 6862, (Mouse) 20997

Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding protein binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development anatomical structure morphogenesis anterior/posterior pattern specification reproductive process notochord development positive regulation of transcription from RNA polymerase II promoter cardiac muscle cell differentiation

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