Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 594

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Published Figures (1)

BrdU Antibody in Immunohistochemistry (IHC)

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BrdU Antibody (B35132) in IHC

Development (Cambridge, England) 2013 - Published figure using BrdU monoclonal antibody (Product # B35132) in Immunohistochemistry

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Product Details

B35132

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Alexa Fluor™ 594 Alexa Fluor™ 594 Alexa Fluor™ 594

Excitation/Emission Max

590/618 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536436

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic impacts on thermostability of onco-lncRNA HOTAIR during the development and progression of endometriosis.

PloS one

Chang CY,Tseng CC,Lai MT,Chiang AJ,Lo LC,Chen CM,Yen MJ,Sun L,Yang L,Hwang T,Tsai FJ,Sheu JJ

B35132 was used in Immunocytochemistry-immunoflourescence to show long non-coding RNA HOTAIR has a prominent role in endometriosis.

Mon Oct 11 00:00:00 EDT 2021

eIF4E S209 phosphorylation licenses myc- and stress-driven oncogenesis.

eLife

Ruan H,Li X,Xu X,Leibowitz BJ,Tong J,Chen L,Ao L,Xing W,Luo J,Yu Y,Schoen RE,Sonenberg N,Lu X,Zhang L,Yu J

B35132 was used in Immunocytochemistry to understand a role of eIF4E S209 in oncogenic translation by generating EIF4ES209A/+ heterozygous knockin (4EKI) HCT 116 human colorectal cancer (CRC) cells.

Mon Nov 02 00:00:00 EST 2020

TGFβ1 Cell Cycle Arrest Is Mediated by Inhibition of MCM Assembly in Rb-Deficient Conditions.

Molecular cancer research : MCR

Nepon-Sixt BS,Alexandrow MG

B35132 was used in Immunoprecipitation to demonstrate that the MCM assembly process is a pivotal target of TGFβ1 in eliciting cell cycle arrest, and provide evidence for a novel oncogenic role for CDT1 in abrogating TGFβ1 inhibition of MCM assembly.

Tue Jan 01 00:00:00 EST 2019

Small-molecule Wnt agonists correct cleft palates in Pax9 mutant mice in utero.

Development (Cambridge, England)

Jia S,Zhou J,Fanelli C,Wee Y,Bonds J,Schneider P,Mues G,D'Souza RN

B35132 was used in Immunofluorescence in immunohistochemistry on frozen tissues to assess the importance of Pax9-dependent Wnt signalling in palatogenesis.

Sun Oct 15 00:00:00 EDT 2017

DEMETER plant DNA demethylase induces antiviral response by interferon signalling in animal cells.

Scientific reports

Mok YG,Choi KY,Hong SH,Huh JH

B35132 was used in Immunocytochemistry-immunoflourescence to investigate whether expression of the plant DEMETER family of 5-methylcytosine DNA glycosylases in human cells allows direct 5-methylcytosine excision.

Tue Aug 22 00:00:00 EDT 2017

Smooth muscle FGF/TGFβ cross talk regulates atherosclerosis progression.

EMBO molecular medicine

Chen PY,Qin L,Li G,Tellides G,Simons M

B35132 was used in Immunocytochemistry-immunoflourescence to examine the contribution of vascular smooth muscle cell contractile-to-proliferative phenotype conversion to atherosclerotic plaque growth.

Fri Jul 01 00:00:00 EDT 2016

Featured Article: Nuclear export of opioid growth factor receptor is CRM1 dependent.

Experimental biology and medicine (Maywood, N.J.)

Kren NP,Zagon IS,McLaughlin PJ

B35132 was used in immunocytochemistry to identify the nuclear export signal for opioid growth factor receptor.

Mon Feb 01 00:00:00 EST 2016

Pax9 regulates a molecular network involving Bmp4, Fgf10, Shh signaling and the Osr2 transcription factor to control palate morphogenesis.

Development (Cambridge, England)

Zhou J,Gao Y,Lan Y,Jia S,Jiang R

B35132 was used in immunocytochemistry to investigate the role of Osr2 and Pax9 in palate development.

Sun Dec 01 00:00:00 EST 2013

Pax9 regulates a molecular network involving Bmp4, Fgf10, Shh signaling and the Osr2 transcription factor to control palate morphogenesis.

Development (Cambridge, England)

Zhou J,Gao Y,Lan Y,Jia S,Jiang R

B35132 was used in immunocytochemistry to investigate the role of Osr2 and Pax9 in palate development.

Sun Dec 01 00:00:00 EST 2013

VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling.

Nature cell biology

Tammela T,Zarkada G,Nurmi H,Jakobsson L,Heinolainen K,Tvorogov D,Zheng W,Franco CA,Murtomäki A,Aranda E,Miura N,Ylä-Herttuala S,Fruttiger M,Mäkinen T,Eichmann A,Pollard JW,Gerhardt H,Alitalo K

B35132 was used in immunohistochemistry to show that macrophage-derived VEGF-C activates VEGFR-3 in tip cells to reinforce Notch signaling.

Sun Sep 11 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 594

BrdU Antibody (B35132) in IHC

Product Details

B35132

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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