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Application
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Brevican Antibody (PA5-47563) in ICC/IF
Immunocytochemistry analysis of Brevican in immersion fixed rat cortical stem cells differentiated for 7 days by growth factor withdrawal. Samples were incubated in Brevican polyclonal antibody (Product # PA5-47563) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
PA5-47563
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Brevican Asp23-Pro911
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606086
Product Specific Information
In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Brevican is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C terminal epitope EAMESE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BEHAB; Brain-enriched hyaluronan-binding protein; brevican (brain specific proteoglycan in the aggrecan family); Brevican core protein; Chondroitin sulfate proteoglycan 7; chondroitin sulfate proteoglycan BEHAB
Gene Aliases: ALPBRE; BCAN; BEHAB; CSPG7; UNQ2525/PRO6018
UniProt ID: (Human) Q96GW7, (Rat) P55068
Entrez Gene ID: (Human) 63827, (Rat) 25393
skeletal system development
cell adhesion
central nervous system development
hippocampus development
extracellular matrix disassembly
extracellular matrix organization
glycosaminoglycan metabolic process
chondroitin sulfate biosynthetic process
chondroitin sulfate catabolic process
dermatan sulfate biosynthetic process
regulation of neuronal synaptic plasticity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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