Loading ...

Invitrogen

C/EBP beta Polyclonal Antibody

3 References

View all (25) C/EBP beta antibodies

Datasheet

Protocols

Questions & Answers

Catalog # PA5-27244

Price (JPY)

57,100

Online offer

Ends: 27-Jun-2025

95,200

Save 38,100 (40%)

100 µL

Join Aspire^™ for free antibodies

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

Application

C/EBP beta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

C/EBP beta Antibody (PA5-27244) in ICC/IF

Immunocytochemistry analysis of C/EBP beta in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Samples were incubated with C/EBP beta polyclonal antibody (Product # PA5-27244) using a dilution of 1:1,000 (green). Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

C/EBP beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C/EBP beta Antibody in Western Blot (WB)

C/EBP beta Antibody in Immunoprecipitation (IP)

C/EBP beta Antibody in ChIP Assay (ChIP)

Product Image Gallery

Product Details

PA5-27244

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

ChIP assay (ChIP)

Assay-Dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP beta. The exact sequence is proprietary.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2544720

Product Specific Information

Recommended positive controls: U87-MG.

Predicted reactivity: Pig (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of a distal enhancer that determines the expression pattern of acute phase marker C-reactive protein.

The Journal of biological chemistry

Wang MY,Zhang CM,Zhou HH,Ge ZB,Su CC,Lou ZH,Zhang XY,Xu TT,Li SY,Zhu L,Zhou YL,Wu Y,Ji SR

PA5-27244 was used in ChIP assay to suggest an intriguing mode of molecular evolution wherein expression-changing mutations in distal regulatory elements initiate subsequent functional selection involving coupling among distal/proximal regulatory mutations and activity-changing coding mutations.

Mon Aug 01 00:00:00 UTC 2022

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

Xian H,Liu Y,Rundberg Nilsson A,Gatchalian R,Crother TR,Tourtellotte WG,Zhang Y,Aleman-Muench GR,Lewis G,Chen W,Kang S,Luevanos M,Trudler D,Lipton SA,Soroosh P,Teijaro J,de la Torre JC,Arditi M,Karin M,Sanchez-Lopez E

PA5-27244 was used in ChIP assay to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 UTC 2021

Long non-coding RNA HOTAIR regulates myeloid differentiation through the upregulation of p21 via miR-17-5p in acute myeloid leukaemia.

RNA biology

Hu L,Liu J,Meng Y,Zheng H,Ding C,Wang H,Charwudzi A,Li M,Li J,Zhai Z,Xiong S

PA5-27244 was used in ChIP assay to provide a novel insight into the mechanism of lncRNA-mediated differentiation and indicate that HOTAIR may be a promising therapeutic target for leukaemia, especially for AML with M2 type.

Fri Oct 01 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: C EBP; C/EBP beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; interleukin 6-dependent DNA-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; Nuclear factor NF-IL6; nuclear factor of interleukin 6; OTTMUSP00000017503; TCF-5; Transcription factor 5; transcription factor C/EBP beta

Gene Aliases: C/EBP-beta; CEBPB; IL6DBP; NF-IL6; PP9092; TCF5

UniProt ID: (Human) P17676

Entrez Gene ID: (Human) 1051

Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding kinase binding histone acetyltransferase binding glucocorticoid receptor binding protein homodimerization activity histone deacetylase binding ubiquitin-like protein ligase binding protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

ovarian follicle development embryonic placenta development granuloma formation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter acute-phase response inflammatory response immune response memory neuron differentiation positive regulation of interleukin-4 production mammary gland epithelial cell proliferation response to endoplasmic reticulum stress T-helper 1 cell activation negative regulation of T cell proliferation defense response to bacterium negative regulation of neuron apoptotic process regulation of interleukin-6 biosynthetic process positive regulation of fat cell differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter brown fat cell differentiation mammary gland epithelial cell differentiation regulation of transcription involved in cell fate commitment intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to interleukin-1 cellular response to organic cyclic compound hepatocyte proliferation liver regeneration positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more