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Invitrogen

C5AR1 Monoclonal Antibody (10/92)

2 Published Figures

3 References

View all (55) C5AR1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (2)

Application

FIGURE: 1 / 4

C5AR1 Antibody (MA1-81761) in Flow

Flow cytometric analysis of mouse peripheral blood granulocytes using a CD88/C5R1 monoclonal antibody (Product # MA1-81763)

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Product Details

MA1-81761

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

10/92

Immunogen

RBL-2H3 transfected cells expressing murine C5aR.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_929568

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR.

Rat anti Mouse CD88 antibody, clone 10/92 recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR.

Target Information

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exogenous inter-α inhibitor proteins prevent cell death and improve ischemic stroke outcomes in mice.

The Journal of clinical investigation

McCullough LD,Roy-O'Reilly M,Lai YJ,Patrizz A,Xu Y,Lee J,Holmes A,Kraushaar DC,Chauhan A,Sansing LH,Stonestreet BS,Zhu L,Kofler J,Lim YP,Venna VR

MA1-81761 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to indicate that IAIP is a potential therapeutic candidate for the treatment of ischemic stroke.

Wed Sep 01 00:00:00 UTC 2021

C5aR1 interacts with TLR2 in osteoblasts and stimulates the osteoclast-inducing chemokine CXCL10.

Journal of cellular and molecular medicine

Mödinger Y,Rapp A,Pazmandi J,Vikman A,Holzmann K,Haffner-Luntzer M,Huber-Lang M,Ignatius A

Published figure using C5AR1 monoclonal antibody (Product # MA1-81761) in Western Blot

Sat Dec 01 00:00:00 UTC 2018

Osteoblast-specific overexpression of complement receptor C5aR1 impairs fracture healing.

PloS one

Bergdolt S,Kovtun A,Hägele Y,Liedert A,Schinke T,Amling M,Huber-Lang M,Ignatius A

Published figure using C5AR1 monoclonal antibody (Product # MA1-81761) in Western Blot

Mon Sep 25 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5a-R; CD88; CD88 antigen; complement component 5, receptor 1

Gene Aliases: C5aR; C5ar1; C5r1; Cd88; D7Msu1

UniProt ID: (Mouse) P30993

Entrez Gene ID: (Mouse) 12273

Function(s)

complement component C5a binding signal transducer activity complement component C5a receptor activity G-protein coupled receptor activity anaphylatoxin receptor activity C5a anaphylatoxin receptor activity G-protein coupled receptor

Process(es)

complement receptor mediated signaling pathway apoptotic process chemotaxis signal transduction G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis cell proliferation in hindbrain neutrophil chemotaxis response to peptidoglycan complement component C5a signaling pathway mRNA transcription from RNA polymerase II promoter negative regulation of neuron apoptotic process positive regulation of angiogenesis positive regulation of epithelial cell proliferation defense response to Gram-positive bacterium positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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