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Invitrogen

CBS Monoclonal Antibody (GT519)

Advanced Verification

2 Published Figures

3 References

View all (21) CBS antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Application

CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

CBS Antibody (MA5-17273) in IHC (P)

Immunohistochemistry (Paraffin) analysis of CBS was performed in paraffin-embedded mouse kidney tissue using CBS Monoclonal Antibody (GT519) (Product # MA5-17273) at a dilution of 1:250.

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CBS Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of CBS was observed in OVCAR-3 when compared to SK-OV-3; Mouse, Rat Liver and Mouse Pancreas when compared to Mouse and Rat Heart using Anti-CBS Monoclonal Antibody (GT519) (Product # MA5-17273) in Western Blot. {RE}

Product Details

MA5-17273

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT519

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538739

Product Specific Information

Recommended positive controls: HeLa, 293T, Raji, K562, mouse liver, rat liver.

Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dexamethasone sensitizes to ferroptosis by glucocorticoid receptor-induced dipeptidase-1 expression and glutathione depletion.

Science advances

von Mässenhausen A,Zamora Gonzalez N,Maremonti F,Belavgeni A,Tonnus W,Meyer C,Beer K,Hannani MT,Lau A,Peitzsch M,Hoppenz P,Locke S,Chavakis T,Kramann R,Muruve DA,Hugo C,Bornstein SR,Linkermann A

MA5-17273 was used in Immunohistochemistry (Paraffin), Western Blot to identify a previously unknown mechanism of glucocorticoid-mediated sensitization to ferroptosis bearing clinical and therapeutic implications.

Fri Feb 04 00:00:00 UTC 2022

Letrozole-Induced Polycystic Ovary Syndrome Attenuates Cystathionine-β Synthase mRNA and Protein Abundance in the Ovaries of Female Sprague Dawley Rats.

The Journal of nutrition

Bries AE,Webb JL,Vogel B,Carrillo C,Keating AF,Pritchard SK,Roslan G,Miller JW,Schalinske KL

MA5-17273 was used in Western Blotting to demonstrate that letrozole-induced PCOS Sprague Dawley rats temporally decrease the ovarian abundance of Cbs mRNA and protein in the early stages of PCOS.

Tue Jun 01 00:00:00 UTC 2021

Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.

Journal of cellular and molecular medicine

Uyy E,Suica VI,Boteanu RM,Safciuc F,Cerveanu-Hogas A,Ivan L,Stavaru C,Simionescu M,Antohe F

MA5-17273 was used in Western Blotting to lead to a novel target in the therapy of diabetic nephropathy.

Thu Oct 01 00:00:00 UTC 2020

Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.

Journal of cellular and molecular medicine

Uyy E,Suica VI,Boteanu RM,Safciuc F,Cerveanu-Hogas A,Ivan L,Stavaru C,Simionescu M,Antohe F

MA5-17273 was used in Western Blotting to lead to a novel target in the therapy of diabetic nephropathy.

Thu Oct 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; cystathionine-beta-synthase; Hemoprotein H-450; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; HIP4

UniProt ID: (Human) P35520, (Rat) P32232, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Rat) 24250, (Mouse) 12411

Function(s)

cystathionine beta-synthase activity cysteine synthase activity protein binding oxygen binding enzyme binding heme binding pyridoxal phosphate binding ubiquitin protein ligase binding identical protein binding protein homodimerization activity metal ion binding nitrite reductase (NO-forming) activity carbon monoxide binding nitric oxide binding modified amino acid binding S-adenosyl-L-methionine binding lyase activity lyase metabolite interconversion enzyme

Process(es)

cysteine biosynthetic process from serine L-serine metabolic process L-serine catabolic process cellular amino acid biosynthetic process cysteine biosynthetic process via cystathionine cysteine biosynthetic process transsulfuration L-cysteine catabolic process DNA protection homocysteine catabolic process homocysteine metabolic process oxidation-reduction process hydrogen sulfide biosynthetic process endochondral ossification blood vessel remodeling superoxide metabolic process cerebellum morphogenesis regulation of cGMP metabolic process response to nutrient levels negative regulation of apoptotic process regulation of JUN kinase activity regulation of blood vessel size response to folic acid maternal process involved in female pregnancy cartilage development involved in endochondral bone morphogenesis cellular response to hypoxia

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