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Proteintech

CBX4 Polyclonal Antibody

4 References

View all (19) CBX4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)

Application

CBX4 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CBX4 Antibody (18544-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 18544-1-AP (CBX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

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Product Details

18544-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:400

View 1 publication 1 publication

in situ PLA (PLA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CBX4 Fusion Protein Ag13425 (1-290 aa encoded by BC014967)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MELPAVGEH VFAVESIEKK RIRKGRVEYL VKWRGWSPKY NTWEPEENIL DPRLLIAFQN RERQEQLMGY RKRGPKPKPL VVQVPTFARR SNVLTGLQDS STDNRAKLDL GAQGKGQGHQ YELNSKKHHH HAVGLNLSHV RKRCLSETHG EREPCKKRLT ARSISTPTCL GGSPAAERPA DLPPAAALPQ PEVILLDSDL DEPIDLRCVK TRSEAGEPPS SLQVKPETPA SAAVAVAAAA APTTTAEKPP AEAQDEPAES LSEFKPFFGN IIITDVTANC LTVTFKEYVT V (1-290 aa encoded by BC014967)

Target Information

Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1). CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Long intergenic noncoding RNA00265 promotes proliferation of gastric cancer via the microRNA-144-3p/Chromobox 4 axis.

Bioengineered

Yang Z,OuYang X,Zheng L,Dai L,Luo W

Wed Dec 01 00:00:00 UTC 2021

SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.

Frontiers in cell and developmental biology

Giordano I,Pirone L,Muratore V,Landaluze E,Pérez C,Lang V,Garde-Lapido E,Gonzalez-Lopez M,Barroso-Gomila O,Vertegaal ACO,Aransay AM,Rodriguez JA,Rodriguez MS,Sutherland JD,Barrio R

Sat Oct 09 00:00:00 UTC 2021

SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.

Frontiers in cell and developmental biology

Giordano I,Pirone L,Muratore V,Landaluze E,Pérez C,Lang V,Garde-Lapido E,Gonzalez-Lopez M,Barroso-Gomila O,Vertegaal ACO,Aransay AM,Rodriguez JA,Rodriguez MS,Sutherland JD,Barrio R

Sat Oct 09 00:00:00 UTC 2021

SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.

Frontiers in cell and developmental biology

Giordano I,Pirone L,Muratore V,Landaluze E,Pérez C,Lang V,Garde-Lapido E,Gonzalez-Lopez M,Barroso-Gomila O,Vertegaal ACO,Aransay AM,Rodriguez JA,Rodriguez MS,Sutherland JD,Barrio R

Sat Oct 09 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: chromobox homolog 4; chromobox homolog 4 (Pc class homolog, Drosophila); Chromobox protein homolog 4; chromobox-like protein 4; E3 SUMO-protein ligase CBX4; E3 SUMO-protein transferase CBX4; hPc2; NS5ATP1-binding protein 16; Pc class 2 homolog; Pc2; Polycomb 2 homolog

Gene Aliases: CBX4; MPc2; NBP16; PC2

UniProt ID: (Human) O00257, (Mouse) O55187

Entrez Gene ID: (Human) 8535, (Mouse) 12418, (Rat) 501403

Function(s)

chromatin binding transcription corepressor activity single-stranded RNA binding protein binding ligase activity SUMO transferase activity enzyme binding SUMO binding methylated histone binding transcription regulatory region DNA binding phosphoprotein binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase protein modifying enzyme

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated covalent chromatin modification protein sumoylation negative regulation of apoptotic process negative regulation of transcription, DNA-templated regulation of transcription, DNA-templated chromatin modification

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CBX4 Polyclonal Antibody

CBX4 Antibody (18544-1-AP) in ICC/IF

Product Details

18544-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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