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Application
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CCDC134 Antibody (MA5-29088) in WB
Western Blot using CCDC134 Recombinant Rabbit Monoclonal Antibody (26) (Product # MA5-29088) at 1:500 dilution. Lane A: HL-60 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.
Product Details
MA5-29088
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
026
Immunogen
Recombinant Human CCDC134 protein (Met1-Leu229)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785016
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CCDC134.
Target Information
The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coiled-coil domain-containing protein 134; dJ821D11.3
Gene Aliases: CCDC134
UniProt ID: (Human) Q9H6E4
Entrez Gene ID: (Human) 79879
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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