Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
FIGURE: 1 / 3
AA Sequence of recombinant protein: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human MIP-1alpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-1alpha (500-P38) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-1alpha.
Western Blot: To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P38BT-1MG will be provided as 2 x 500 µg
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support