Invitrogen

CCL3 Monoclonal Antibody (756613)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (37) CCL3 (MIP-1 alpha) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CCL3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

CCL3 Antibody (MA5-24364) in ICC/IF

Immunocytochemistry analysis of CCL3 in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line stimulated (upper panel) or not (lower panel) with LPS. Samples were incubated in CCL3 monoclonal antibody (Product # MA5-24364) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of stimulated cells.

Product Image Gallery

CCL3 Antibody — Figure 7 Role of fisetin in regulating the CXCLs signaling pathway in mice with PRI-induced systemic lupus erythematosus. (A) Western blot analysis was used to determine the expression levels of CXCL-1, CXCL-2, CCL-3 and CXCR-2 in the spleen tissue samples obtained from mice subjected to various treatments. Representative images are provided. (B) Quantification of CXCL-1, CXCL-2, CCL-3 and CXCR-2 protein levels from the western blots. Values are expressed as the mean +- standard error of the mean (n=10 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the PRI group. Con, control; PRI, pristine; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F25/50/100, treatment with 25/50/100 mg/kg fisetin. {TM}

Product Details

MA5-24364

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

8-25 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

756613

Immunogen

E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609517

Product Specific Information

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation.

International journal of molecular medicine

Xu SP,Li YS

Published figure using CCL3 monoclonal antibody (Product # MA5-24364) in Western Blot

Sat Dec 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: C-C motif chemokine 3; H-MIP-1-alpha; Heparin-binding chemotaxis protein; L2G25B; LD78-alpha; Macrophage inflammatory protein 1-alpha; macrophage inflammatory protein-1alpha; MIP-1 alpha; MIP-1-alpha; MIP1 (a); mip1 alpha; RP23-320E6.7; SIS-alpha; small inducible cytokine A3; Small-inducible cytokine A3; TY-5

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Gene Aliases: AI323804; Ccl3; G0S19-1; LD78alpha; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; Scya3

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UniProt ID: (Mouse) P10855

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Entrez Gene ID: (Mouse) 20302

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Function(s)

cytokine activity protein binding chemokine activity cytokine intercellular signal molecule

Process(es)

chemotaxis inflammatory response immune response positive regulation of cytosolic calcium ion concentration regulation of cell shape neutrophil chemotaxis leukocyte chemotaxis positive regulation of tumor necrosis factor production positive regulation of neuron apoptotic process astrocyte cell migration positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter macrophage chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response cell chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCL3 Monoclonal Antibody (756613)

CCL3 Antibody (MA5-24364) in ICC/IF

Product Details

MA5-24364

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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