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            • Primary Antibodies ›
            • CD11b Antibodies

            Invitrogen

            CD11b Monoclonal Antibody (ICRF44), APC, eBioscience™

            36 Published Figures
            51 References
            View all (253) CD11b antibodies
            Datasheet
            Protocols
            Questions & Answers
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            Catalog # 17-0118-42
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            Ends: 01-Jul-2025
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            Cite CD11b Monoclonal Antibody (ICRF44), APC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (36)
            Application
            CD11b Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 37

            CD11b Antibody (17-0118-42) in Flow

            Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD11b APC (purple histogram). Cells in the monocyte (left) or granulocyte (right) gate were used for analysis.
            View Product

            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)
            CD11b Antibody in Flow Cytometry (Flow)

            Product Details

            17-0118-42

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            5 µL (0.5 µg)/test
            View 51 publications 51 publications
            Product Specifications

            Species Reactivity

            Baboon, Chimpanzee, Cynomolgus monkey, Human, Rhesus monkey

            Published species

            Cynomolgus monkey, Human, Mouse, Pig

            Host/Isotype

            Mouse / IgG1, kappa

            Recommended Isotype Control

            Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            ICRF44

            Conjugate

            APC
            • Unconjugated
            • Alexa Fluor 700 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 506
            • FITC
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 800
            • NovaFluor Red 710
            • NovaFluor Red 725
            • NovaFluor Red 755
            • NovaFluor Violet 690
            • NovaFluor Violet 800
            • NovaFluor Yellow 570
            • NovaFluor Yellow 590
            • NovaFluor Yellow 660
            • NovaFluor Yellow 755
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            651/660 nm View spectra spectra

            Form

            Liquid

            Concentration

            5 µL/Test

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2, with BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_2016659

            Product Specific Information

            Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.

            Applications Reported: This ICRF44 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This ICRF44 antibody has been pre-titrated and tested by flow cytometric analysis of normal human blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

            Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: antigen CD11b (p170); CD11 antigen-like family member B; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; complement component 3 receptor 3 subunit; CR-3 alpha chain; integrin alpha M; Integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); Leukocyte adhesion receptor MO1; Mac-1 alpha (Mac1A); MAC-1 alpha subunit; macrophage antigen alpha polypeptide; MGC117044; Neutrophil adherence receptor; neutrophil adherence receptor alpha-M subunit; unnamed protein product

            View more View less

            Gene Aliases: CD11B; CR3A; ITGAM; MAC-1; MAC1A; MO1A; SLEB6

            View more View less

            UniProt ID: (Human) P11215

            View more View less

            Entrez Gene ID: (Human) 3684, (Rhesus monkey) 714832, (Cynomolgus monkey) 101866035, (Chimpanzee) 454069

            View more View less

            Function(s)

            glycoprotein binding protein binding metal ion binding integrin

            Process(es)

            cell adhesion integrin-mediated signaling pathway ectodermal cell differentiation extracellular matrix organization toll-like receptor 4 signaling pathway leukocyte migration
            It has to be done as per old AB suggested Products section.
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