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Invitrogen
CD133 Monoclonal Antibody (5E3)
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Application
FIGURE: 1 / 6
CD133 Antibody (MA5-18323) in WB
Western Blot was performed using Anti-CD133 Monoclonal Antibody (5E3) (Product # MA5-18323) and a 133 kDa band corresponding to Prominin-1 was observed across cell lines tested except HeLa and SK-O-V3 which are reported to be low or negative for CD133 expression. Membrane enriched extracts (30 µg lysate) of NTERA-2 cl.D1 (Lane 1), Caco-2 (Lane 2), HT-29 (Lane 3), HeLa (Lane 4) and SK-O-V3 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulo... View More
Product Details
MA5-18323
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
5E3
Immunogen
Recombinant full length human CD133
Conjugate
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539686
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 133 kDa and does not cross-react with related proteins.
Purity is >95% by SDS-PAGE.
Target Information
The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Antigen AC133; antigen CD133; CD107b; CD133; hematopoietic stem cell antigen; hProminin; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp2; LGP-96; LGP-B; Mac3; Prominin-1; Prominin-like protein 1; Prominin1
Gene Aliases: AC133; CD133; CORD12; MCDR2; MSTP061; PROM1; PROML1; RP41; STGD4
UniProt ID: (Human) O43490
Entrez Gene ID: (Human) 8842
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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