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Invitrogen
CD134 (OX40) Monoclonal Antibody (OX40), APC, eBioscience™
FIGURE: 1 / 1
CD134 (OX40) Antibody (17-1342-82) in Flow
Staining of 3-day Con A-stimulated rat splenocytes with Anti-Rat CD4 PE (Product # 12-0040-82) and 0.06 µg of Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or 0.06 µg of Anti-Rat CD134 (OX40) APC (right). Total viable cells were used for analysis.
Product Details
17-1342-82
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX40
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573160
Product Specific Information
Description: The monoclonal antibody OX40 reacts with rat CD134, a 50-kDa glycoprotein belonging to the TNFR superfamily. CD134, which is also known as OX40, is expressed on activated T cells, TH1, TH2, TH17, and Tregs, but not on naive CD4 or CD8 T cells or most memory cells. CD134 is also inducible on NKT cells, neutrophils, and NK cells. CD134 binds to CD252 (OX40L) to mediate T cell expansion and survival, as well as memory T cell generation. Finally, CD134 is involved in Treg suppression and tolerance.
Applications Reported: This OX40 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX40 antibody has been tested by flow cytometric analysis of stimulated rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
OX40 is a protein receptor found on the surface of T cells, a type of white blood cell that plays a crucial role in the immune system. When activated, OX40 promotes the proliferation and survival of T cells, leading to a stronger immune response against cancer cells and infectious agents. OX40 agonists, which are drugs that activate OX40, have shown promising results in preclinical and clinical studies as a potential immunotherapy for cancer. They can enhance the efficacy of other cancer treatments such as chemotherapy and checkpoint inhibitors, and may also have applications in autoimmune diseases and allergies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD134; MRC OX40; OX40 antigen; OX40L receptor; Tax-transcriptionally activated glycoprotein 1; tumor necrosis factor (ligand) superfamily member 4; Tumor necrosis factor receptor superfamily member 4; tumor necrosis factor superfamily, member 4
Gene Aliases: Ox40; Tnfrsf4; Txgp1; Txgp1l
UniProt ID: (Rat) P15725
Entrez Gene ID: (Rat) 25572
inflammatory response
cellular defense response
programmed cell death
positive regulation of B cell proliferation
tumor necrosis factor-mediated signaling pathway
T cell proliferation
regulation of apoptotic process
negative regulation of sequence-specific DNA binding transcription factor activity
regulation of protein kinase activity
negative regulation of transcription, DNA-templated
negative regulation of cytokine secretion
positive regulation of immunoglobulin secretion
negative regulation of activation-induced cell death of T cells
negative regulation of extrinsic apoptotic signaling pathway
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