Invitrogen

CD14 Monoclonal Antibody (TuK4), PE

4 Published Figures

9 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

FIGURE: 1 / 5

CD14 Antibody (MHCD1404) in Flow

Human peripheral blood monocytes stained using R-PE of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

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Product Details

MHCD1404

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 10% sucrose, 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373109

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

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Product References

miR-181a Modulation of ERK-MAPK Signaling Sustains DC-SIGN Expression and Limits Activation of Monocyte-Derived Dendritic Cells.

Cell reports

Lim CX,Lee B,Geiger O,Passegger C,Beitzinger M,Romberger J,Stracke A,Högenauer C,Stift A,Stoiber H,Poidinger M,Zebisch A,Meister G,Williams A,Flavell RA,Henao-Mejia J,Strobl H

MHCD1404 was used in Flow cytometry/Cell sorting to demonstrate that high DC-SIGN expression levels and a high activation threshold in monocyte-derived dendritic cells are linked and simultaneously maintained by miR-181a.

Tue Mar 17 00:00:00 EDT 2020

Physical exercise and human adipose-derived mesenchymal stem cells ameliorate motor disturbances in a male rat model of Parkinson's disease.

Journal of neuroscience research

Cucarián JD,Berrío JP,Rodrigues C,Zancan M,Wink MR,de Oliveira A

Published figure using CD14 monoclonal antibody (Product # MHCD1404) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.

Cancer immunology research

Weiskopf K,Anderson KL,Ito D,Schnorr PJ,Tomiyasu H,Ring AM,Bloink K,Efe J,Rue S,Lowery D,Barkal A,Prohaska S,McKenna KM,Cornax I,O'Brien TD,O'Sullivan MG,Weissman IL,Modiano JF

MHCD1404-4 was used in Flow cytometry/Cell sorting to demonstrate, using a mouse xenograft model, the effectiveness of combining CD47-blockade with anti-CD20 as treatment against canine lymphoma.

Thu Dec 01 00:00:00 EST 2016

Analysis of the safety of mesenchymal stromal cells secretome for glioblastoma treatment.

Cytotherapy

Onzi GR,Ledur PF,Hainzenreder LD,Bertoni AP,Silva AO,Lenz G,Wink MR

MHCD1404 was used in flow cytometry to assess the use of human adipose-derived stem cells to treat cancer

Fri Jul 01 00:00:00 EDT 2016

Antibacterial responses by peritoneal macrophages are enhanced following vitamin D supplementation.

PloS one

Bacchetta J,Chun RF,Gales B,Zaritsky JJ,Leroy S,Wesseling-Perry K,Boregaard N,Rastogi A,Salusky IB,Hewison M

MHCD1404 was used in Flow cytometry/Cell sorting to show that vitamin D supplementation in vitro and in vivo promotes innate immune responses that may enhance macrophage antibacterial responses in patients undergoing PD.

Fri Aug 28 00:00:00 EDT 2015

Coexpression of CD14 and CD326 discriminate hepatic precursors in the human fetal liver.

Stem cells and development

Fomin ME,Tai LK,Bárcena A,Muench MO

Published figure using CD14 monoclonal antibody (Product # MHCD1404) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2011

The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Journal of cellular and molecular medicine

Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R

Thu Apr 01 00:00:00 EDT 2010

Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma.

The Journal of pathology

Strickland LA,Ross J,Williams S,Ross S,Romero M,Spencer S,Erickson R,Sutcliffe J,Verbeke C,Polakis P,van Bruggen N,Koeppen H

MHCD1404 was used in flow cytometry to investigate targeting CEACAM6 for treatment of pancreatic adenocarcinomas.

Wed Jul 01 00:00:00 EDT 2009

Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy.

Journal of immunological methods

Canonico B,Zamai L,Burattini S,Granger V,Mannello F,Gobbi P,Felici C,Falcieri E,Reilly JT,Barnett D,Papa S

MHCD1404 was used in flow cytometry to assess the effects of a TransFix-based stabilization technique on leukocyte scatter characteristics, immunophenotyping, membrane permeability, absolute cell counting and morphology.

Wed Dec 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

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CD14 Monoclonal Antibody (TuK4), PE

CD14 Antibody (MHCD1404) in Flow

Product Details

MHCD1404

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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