Invitrogen

CD147 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

13 References

View all (111) CD147 antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

CD147 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

CD147 Antibody (34-5600) in ICC/IF

Immunofluorescent analysis of CD147/BSG was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD147/BSG Rabbit Polyclonal Antibody (Product # 34-5600) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More

CD147 Antibody in Western Blot, ELISA (WB, ELISA)

Product Image Gallery

CD147 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in CD147 knockout (KO) cell line when compared to control cell line using anti-CD147 antibody (Product # 34-5600). {KO}

Product Details

34-5600

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (IHC)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the N-terminal region of the human EMMPRIN protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533176

Target Information

CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6, EMMPRIN, extracellular matrix metalloproteinase inducer, TCSF, tumour cell-derived collagenase-stimulatory factor) is a 50-60 kDa glycoprotein with multiple glycosylated forms. CD147 is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. CD147 is also a member of the immunoglobulin superfamily. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Uptake of severe acute respiratory syndrome coronavirus 2 spike protein mediated by angiotensin converting enzyme 2 and ganglioside in human cerebrovascular cells.

Frontiers in neuroscience

McQuaid C,Solorzano A,Dickerson I,Deane R

34-5600 was used in Immunocytochemistry-immunofluorescence to suggest that in addition to ACE2, gangliosides are also an important entry point of SARS-CoV-2/SP into these cells.

Tue Mar 07 00:00:00 EST 2023

Regulation of Hook1-mediated endosomal sorting of clathrin-independent cargo by γ-taxilin.

Journal of cell science

Higashi S,Makiyama T,Sakane H,Nogami S,Shirataki H

34-5600 was used in Western Blotting to suggest that γ-taxilin negatively regulates the sorting of Hook1-mediated cargo proteins into recycling endosomes by interfering with the interactions between Hook1 and the cargo proteins.

Thu Dec 01 00:00:00 EST 2022

Hydroxypropyl-beta-cyclodextrin (HP-BCD) inhibits SARS-CoV-2 replication and virus-induced inflammatory cytokines.

Antiviral research

Bezerra BB,Silva GPDD,Coelho SVA,Correa IA,Souza MRM,Macedo KVG,Matos BM,Tanuri A,Matassoli FL,Costa LJD,Hildreth JEK,Arruda LB

34-5600 was used in Western Blotting to indicate that the use hydroxypropyl-beta-cyclodextrin (HP-BCD), which inhibits both SARS-CoV2 replication and production of proinflammatory cytokines, as a potential COVID-19 therapeutic warrants further investigation.

Thu Sep 01 00:00:00 EDT 2022

CD147-targeted siRNA in A375 malignant melanoma cells induces the phosphorylation of EGFR and downregulates cdc25C and MEK phosphorylation.

Oncology letters

Hatanaka M,Higashi Y,Kawai K,Su J,Zeng W,Chen X,Kanekura T

34-5600 was used in western blot to analyze the induction of the phosphorylation of EGFR and downregulation of cdc25C and MEK phosphorylation by CD147-targeted siRNA in A375 malignant melanoma cells

Fri Apr 01 00:00:00 EDT 2016

EMMPRIN co-expressed with matrix metalloproteinases predicts poor prognosis in patients with osteosarcoma.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Futamura N,Nishida Y,Urakawa H,Kozawa E,Ikuta K,Hamada S,Ishiguro N

34-5600 was used in immunohistochemistry - paraffin section to evaluate the prognostic significance of EMMPRIN and matrix metalloproteinases for osteosarcoma

Sun Jun 01 00:00:00 EDT 2014

Extracellular matrix metalloproteinase inducer shows active perivascular cuffs in multiple sclerosis.

Brain : a journal of neurology

Agrawal SM,Williamson J,Sharma R,Kebir H,Patel K,Prat A,Yong VW

34-5600 was used in immunohistochemistry - frozen section to examine the expression of extracellular matrix metalloproteinase inducer in murine experimental autoimmune encephalomyelitis and multiple sclerosis specimens.

Sat Jun 01 00:00:00 EDT 2013

Renal cell neoplasias: reversion-inducing cysteine-rich protein with Kazal motifs discriminates tumor subtypes, while extracellular matrix metalloproteinase inducer indicates prognosis.

Journal of translational medicine

Rabien A,Stephan C,Kilic E,Weichert W,Kristiansen G,Miller K,Jung K,Erbersdobler A

34-5600 was used in immunohistochemistry - paraffin section and western blot to study reversion-inducing cysteine-rich protein with Kazal motifs and the extracellular matrix metalloproteinase inducer in renal cell cancer.

Wed Oct 16 00:00:00 EDT 2013

High extracellular matrix metalloproteinase inducer/CD147 expression is strongly and independently associated with poor prognosis in colorectal cancer.

Human pathology

Stenzinger A,Wittschieber D,von Winterfeld M,Goeppert B,Kamphues C,Weichert W,Dietel M,Rabien A,Klauschen F

34-5600 was used in immunohistochemistry to examine expression of extracellular matrix metalloproteinase inducer in patients with colorectal cancer.

Sat Sep 01 00:00:00 EDT 2012

Decreased RECK and Increased EMMPRIN expression in urothelial carcinoma of the bladder are associated with tumor aggressiveness.

Pathobiology : journal of immunopathology, molecular and cellular biology

Wittschieber D,Stenzinger A,Klauschen F,Stephan C,Jung K,Erbersdobler A,Rabien A

Wed Nov 02 00:00:00 EDT 2011

Multi-photon imaging of tumor cell invasion in an orthotopic mouse model of oral squamous cell carcinoma.

Journal of visualized experiments : JoVE

Gatesman Ammer A,Hayes KE,Martin KH,Zhang L,Spirou GA,Weed SA

34-5600 was used in immunohistochemistry - paraffin section to describe a protocol that allows multi-vectorial visualization of lingual tumor spread.

Mon Jul 25 00:00:00 EDT 2011

Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.

American journal of physiology. Heart and circulatory physiology

Venkatesan B,Valente AJ,Reddy VS,Siwik DA,Chandrasekar B

Published figure using CD147 polyclonal antibody (Product # 34-5600) in ELISA

Sat Aug 01 00:00:00 EDT 2009

Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.

American journal of physiology. Heart and circulatory physiology

Venkatesan B,Valente AJ,Reddy VS,Siwik DA,Chandrasekar B

Published figure using CD147 polyclonal antibody (Product # 34-5600) in ELISA

Sat Aug 01 00:00:00 EDT 2009

EMMPRIN modulates migration and deposition of TN-C in oral squamous carcinoma.

Anticancer research

Dang D,Atakilit A,Ramos DM

34-5600 was used in western blot to investigate the contribution of extracellular matrix metalloproteinase inducer to tumor development

Tue Sep 16 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: 5F7; Basigin; CD147; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; HAb18G; Hepatoma-associated antigen; Leukocyte activation antigen M6; OK blood group antigen; TCSF; Tumor cell-derived collagenase stimulatory factor

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Gene Aliases: 5F7; BSG; CD147; EMMPRIN; OK; TCSF; UNQ6505/PRO21383

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UniProt ID: (Human) P35613

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Entrez Gene ID: (Human) 682

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Function(s)

protein binding mannose binding monocarboxylic acid transmembrane transporter activity cadherin binding involved in cell-cell adhesion scaffold/adaptor protein

Process(es)

pyruvate metabolic process cell surface receptor signaling pathway embryo implantation monocarboxylic acid transport extracellular matrix disassembly extracellular matrix organization odontogenesis of dentin-containing tooth response to peptide hormone response to mercury ion decidualization leukocyte migration response to cAMP protein targeting to plasma membrane cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD147 Polyclonal Antibody

CD147 Antibody (34-5600) in ICC/IF

Product Details

34-5600

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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