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Invitrogen
CD150 Monoclonal Antibody (9D1), APC, eBioscience™
Application
FIGURE: 1 / 2
CD150 Antibody (17-1501-81) in Flow
Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.5 µg of Rat IgG1 kappa Isotype Control APC (Product # 17-4301-82) (left) or 0.5 µg of Anti-Mouse CD150 APC (right). Total viable cells were used for analysis.
Product Details
17-1501-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
9D1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469441
Product Specific Information
Description: The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein also known as Signaling Lymphocyte Activation Molecule (SLAM). CD150 is expressed by T (especially TH1) and B cells and its expression is rapidly upregulated on these cells upon activation. Immature thymocytes and dendritic cells also express this antigen. Signaling through SLAM in T cells induces proliferation and augmentation of the interferon-gamma response. Furthermore, SLAM is thought to play a role in adhesion between the T cell and antigen-presenting cell. 9D1 is reported to be an activating antibody.
Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers, such as CD150+CD244-CD48-. For this application we recommend the use of antibody clone mShad150 (Product # 12-1502).
Applications Reported: This 9D1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 9D1 antibody has been tested by flow cytometric analysis of mouse thymic and splenic cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD150, also known as SLAM (Signaling Lymphocyte Activation Molecule), is a member of the immunoglobulin gene superfamily and plays a crucial role in T cell stimulation and immune cell interactions. It is constitutively expressed on peripheral blood memory T cells, T cell clones, immature thymocytes, and a proportion of B cells. CD150 expression is rapidly upregulated upon activation, particularly on naive T cells, Th1 cells, and activated B cells, which express the membrane-bound, soluble, and cytoplasmic isoforms of SLAM. In T cells, signaling through SLAM induces proliferation and enhances the interferon-gamma response. SLAM is also involved in adhesion between T cells and antigen-presenting cells, facilitating immune cell interactions. Homophilic SLAM-SLAM binding during B to B and B to T cell interactions is suggested to enhance the expansion and differentiation of activated B cells. CD150 serves as a receptor for the measles virus and acts as a co-activator on T and B cells. It interacts with SH2D1A and PTPN11 via its cytoplasmic domain, playing a role in signal transduction. Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP), highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD150; CD150 antigen; signaling lymphocyte activation molecule; Signaling lymphocytic activation molecule; SLAM family member 1
Gene Aliases: 4933415F16; AA177906; CD150; CDw150; ESTM51; IPO-3; Slam; Slamf1
UniProt ID: (Mouse) Q9QUM4
Entrez Gene ID: (Mouse) 27218
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