Invitrogen

CD16 Monoclonal Antibody (3G8), Pacific Orange™

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FIGURE: 1 / 1

CD16 Antibody (MHCD1630) in Flow

Human peripheral blood lymphocytes were stained with Pacific Orange™-conjugated anti-human CD16 monoclonal antibody (Product # MHCD1630). The negative profile represents unstained cells.

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Product Details

MHCD1630

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3G8

Immunogen

Human CD16

Conjugate

Pacific Orange™ Pacific Orange™ Pacific Orange™

Excitation/Emission Max

400/551 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374733

Product Specific Information

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

CD16 encodes a receptor that recognizes the Fc portion of immunoglobulin G and is involved in the clearance of immune complexes from the circulation, as well as other functions such as cellular mediated cytotoxicity and enhancement of virus infections. This gene, FCGR3A, shares a high degree of similarity with another nearby gene, FCGR3B, located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, while FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been associated with immunodeficiency 20 and have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Diseases associated with FCGR3A include Immunodeficiency 20 and Herpes Zoster.

Analyte Specific Reagent

How to use the Panel Builder

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Product References

Respiratory Syncytial Virus Infects Primary Neonatal and Adult Natural Killer Cells and Affects Their Antiviral Effector Function.

The Journal of infectious diseases

van Erp EA,Feyaerts D,Duijst M,Mulder HL,Wicht O,Luytjes W,Ferwerda G,van Kasteren PB

MHCD1630 was used in Flow cytometry/Cell sorting to highlight the importance of understanding the interactions between innate effector cells and virus-specific antibodies.

Fri Feb 15 00:00:00 EST 2019

Expression Profile of Human Fc Receptors in Mucosal Tissue: Implications for Antibody-Dependent Cellular Effector Functions Targeting HIV-1 Transmission.

PloS one

Cheeseman HM,Carias AM,Evans AB,Olejniczak NJ,Ziprin P,King DF,Hope TJ,Shattock RJ

MHCD1630 was used in Flow cytometry/Cell sorting to show that the majority of effector cells across the different mucosal locations reside within the subepithelial lamina propria.

Wed Jul 05 00:00:00 EDT 2017

Utilility of flow cytometry as ancillary study to improve the cytologic diagnosis of thyroid lymphomas.

Cytometry. Part B, Clinical cytometry

Stacchini A,Pacchioni D,Demurtas A,Aliberti S,Cassenti A,Isolato G,Gazzera C,Veltri A,Sapino A,Papotti M,Freddi M,Palestini N,Sisto G,Novero D

MHCD1630 was used in flow cytometry to assess the efficacy of using flow cytometry immunophenotyping with fine-needle aspiration cytology for the diagnosis of thyroid lymphoma.

Wed May 25 00:00:00 EDT 2016

LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lee DJ,Sieling PA,Ochoa MT,Krutzik SR,Guo B,Hernandez M,Rea TH,Cheng G,Colonna M,Modlin RL

MHCD1630 was used in flow cytometry to test if leukocyte Ig-like receptor A2 regulates DC differentiation using a leprosy model.

Sat Dec 15 00:00:00 EST 2007

Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.

Journal of immunology (Baltimore, Md. : 1950)

Steinberger P,Majdic O,Derdak SV,Pfistershammer K,Kirchberger S,Klauser C,Zlabinger G,Pickl WF,Stöckl J,Knapp W

MHCD1630 was used in flow cytometry to characterize B7-H3.

Sun Feb 15 00:00:00 EST 2004

B7-H1 (programmed death-1 ligand) on dendritic cells is involved in the induction and maintenance of T cell anergy.

Journal of immunology (Baltimore, Md. : 1950)

Selenko-Gebauer N,Majdic O,Szekeres A,Höfler G,Guthann E,Korthäuer U,Zlabinger G,Steinberger P,Pickl WF,Stockinger H,Knapp W,Stöckl J

MHCD1630 was used in ELISA to identify immunoregulatory molecules on dendritic cells.

Tue Apr 01 00:00:00 EST 2003

Prospective isolation of human clonogenic common myeloid progenitors.

Proceedings of the National Academy of Sciences of the United States of America

Manz MG,Miyamoto T,Akashi K,Weissman IL

MHCD1630 was used in flow cytometry to report how to isolate highly purified hematopoietic intermediates.

Tue Sep 03 00:00:00 EDT 2002

Prognostic significance of CD56 antigen expression in acute myeloid leukemia.

Haematologica

Di Bona E,Sartori R,Zambello R,Guercini N,Madeo D,Rodeghiero F

MHCD1630 was used in flow cytometry to examine CD56 expression in a cohort of acute myeloid leukemia patients in to assess its frequency and prognostic relevance.

Fri Mar 01 00:00:00 EST 2002

Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.

Blood

Sharron M,Pöhlmann S,Price K,Lolis E,Tsang M,Kirchhoff F,Doms RW,Lee B

MHCD1630 was used in flow cytometry to characterize the localization and function of STRL33/Bonzo.

Sat Jul 01 00:00:00 EDT 2000

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW

MHCD1630 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.

Tue Apr 27 00:00:00 EDT 1999

Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions.

The Journal of experimental medicine

Ravetch JV,Perussia B

MHCD1630 was used in flow cytometry and immunoprecipitation to compare Fc gamma RIII(CD16) transcripts isolated from PMN and NK cells.

Tue Aug 01 00:00:00 EDT 1989

Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG.

Proceedings of the National Academy of Sciences of the United States of America

Peltz GA,Grundy HO,Lebo RV,Yssel H,Barsh GS,Moore KW

MHCD1630 was used in immunoprecipitation to identify a locus for at least two Fc gamma R genes on human chromosome 1.

Wed Feb 01 00:00:00 EST 1989

Bioinformatics

Protein Aliases: CD16-I; CD16-II; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; FCG2; FcgammaRIIIA; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; IgG Fc receptor III-1; IgG Fc receptor III-2; IgG Fc receptor III-A; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; neutrophil-specific antigen NA; RP11-5K23.1

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Gene Aliases: CD16; CD16A; CD16B; FCG3; FCGR3; FCGR3A; FCGR3B; FCGRIII; FCR-10; FCRIII; FCRIIIA; FCRIIIb; IGFR3; IMD20

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UniProt ID: (Human) P08637, (Human) O75015

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Entrez Gene ID: (Human) 2214, (Human) 2215

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Function(s)

IgG binding immunoglobulin receptor superfamily

Process(es)

immune response Fc-gamma receptor signaling pathway involved in phagocytosis regulation of immune response positive regulation of defense response to virus by host mitophagy in response to mitochondrial depolarization xenophagy

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CD16 Monoclonal Antibody (3G8), Pacific Orange™

CD16 Antibody (MHCD1630) in Flow

Product Details

MHCD1630

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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