Invitrogen

CD178 (Fas Ligand) Monoclonal Antibody (MFL3), APC, eBioscience™

CD178 (Fas Ligand) Antibody in Flow Cytometry (Flow)

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CD178 (Fas Ligand) Antibody (17-5911-82) in Flow

Staining of mouse CD178-transfected cells with 0.125 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD178 (Fas Ligand) APC (purple histogram). Total viable cells were used for analysis.

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Product Details

17-5911-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MFL3

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717074

Product Specific Information

Description: The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Tested: This MFL3 antibody has been tested by flow cytometric analysis of mouse FasL-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD178 (Fas ligand, FasL) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor, FasR, is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. As a member of the TNF-cytokine family CD178 induces apoptosis when interacting with FasR. CD178 may exist as either membrane bound (45 kD) or soluble forms (26 kD). The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of CD178 to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins. CD178 is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. CD178 expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney. It is assumed that induction of apoptosis through CD178 is predominantly involved in anti-viral immune responses. CD178 is a cell surface molecule belonging to the tumor necrosis factor family, binds to its receptor Fas, thus inducing apoptosis. Various cells express FAS, where CD178 is expressed predominantly on activated T cells. FAS and CD178 are involved in the down-regulation of immune reactions as well as T cell-mediated cytotoxicity. CD178 concentration has also been shown to be associated with atherosclerosis and inflammatory disease, in patients with hypertension. The Fas/ CD178 system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 17-5911-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

17-5911-82 was used in Flow Cytometry to present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells.

Fri Dec 17 00:00:00 EST 2021

A macrophage-T cell coculture model for severe tissue injury-induced T cell death.

STAR protocols

Zhu J,Cao J,Liesz A,Roth S

17-5911-82 was used in Flow Cytometry to present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells.

Fri Dec 17 00:00:00 EST 2021

CD160 serves as a negative regulator of NKT cells in acute hepatic injury.

Nature communications

Kim TJ,Park G,Kim J,Lim SA,Kim J,Im K,Shin MH,Fu YX,Del Rio ML,Rodriguez-Barbosa JI,Yee C,Suh KS,Kim SJ,Ha SJ,Lee KM

17-5911-82 was used in Flow cytometry/Cell sorting to show that CD160 is not essential for NKT cell development.

Mon Jul 22 00:00:00 EDT 2019

Cancer-associated fibroblasts induce antigen-specific deletion of CD8 ⁺ T Cells to protect tumour cells.

Nature communications

Lakins MA,Ghorani E,Munir H,Martins CP,Shields JD

17-5911 was used in Immunohistochemistry-immunofluorescence to examine the immune checkpoint-mediated role of cancer-associated fibroblasts in suppressing T cell activity within the tumour microenvironment.

Mon Mar 05 00:00:00 EST 2018

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 17-5911-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Hypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.

Journal of the American Society of Nephrology : JASN

Zhang J,Han C,Dai H,Hou J,Dong Y,Cui X,Xu L,Zhang M,Xia Q

Published figure using CD178 (Fas Ligand) monoclonal antibody (Product # 17-5911-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Angiotensin converting enzyme inhibitors and angiotensin II receptor antagonist attenuate tumor growth via polarization of neutrophils toward an antitumor phenotype.

Oncoimmunology

Shrestha S,Noh JM,Kim SY,Ham HY,Kim YJ,Yun YJ,Kim MJ,Kwon MS,Song DK,Hong CW

17-5911 was used in Flow cytometry/Cell sorting to investigate how modulation of Ang II pathway attenuates tumour growth via polarisation of neutrophils to an antitumoural phenotype.

Tue Jan 12 00:00:00 EST 2021

Activated T cell exosomes promote tumor invasion via Fas signaling pathway.

Journal of immunology (Baltimore, Md. : 1950)

Cai Z,Yang F,Yu L,Yu Z,Jiang L,Wang Q,Yang Y,Wang L,Cao X,Wang J

17-5911 was used in Flow cytometry/Cell sorting to investigate the effect of FasL-containing exosomes on cell apoptosis in tumours, showing that they promote tumour invasion.

Fri Jun 15 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Apoptosis (APO 1) antigen ligand 1; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); CD95-L; Fas antigen ligand; Fas ligand; Fas-LG; generalized lymphoproliferative disease; soluble form; TNFL; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor ligand; Tumor necrosis factor ligand superfamily member 6; Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

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Gene Aliases: APT1LG1; CD178; CD95-L; CD95L; Fas-L; Fasl; Faslg; gld; Tnfsf6

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UniProt ID: (Mouse) P41047

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Entrez Gene ID: (Mouse) 14103

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Function(s)

death receptor binding cytokine activity tumor necrosis factor receptor binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated regulation of transcription, DNA-templated apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory cell apoptotic process immune response positive regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors negative regulation of angiogenesis cellular chloride ion homeostasis positive regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of epidermal growth factor receptor signaling pathway retinal cell programmed cell death endosomal lumen acidification T cell apoptotic process necroptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway positive regulation of endothelial cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD178 (Fas Ligand) Monoclonal Antibody (MFL3), APC, eBioscience™

CD178 (Fas Ligand) Antibody (17-5911-82) in Flow

Product Details

17-5911-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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