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Invitrogen

CD19 Monoclonal Antibody (SJ25-C1), Pacific Blue™

Advanced Verification

3 Published Figures

27 References

View all (353) CD19 antibodies

Datasheet

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Catalog # MHCD1928

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442.65

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500 µL

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Datasheet

Protocols

Questions & Answers

Published Figures (3)
Advanced Verification (1)

Application

FIGURE: 1 / 4

CD19 Antibody (MHCD1928) in Flow

The Journal of clinical investigation 2024 - Loss of CD19 expression in CD19 CAR-T-treated pediatric patients with ALL corresponds to a loss of CD22 expression. ( A and B ) Reduced CD19 and CD22 expression on resistant cells seen by FACS analysis. Gating was based on the isotype control. ( C and D ) CD19 and CD22 antigen expression was observed in a cohort of cells from pediatric patients with ALL ( n = 11) before treatment and after relapse following... View More

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

MHCD1928

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 22 publications 22 publications

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SJ25-C1

Immunogen

Nalm 1 Human cell line

View immunogen

Conjugate

Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10373689

Product Specific Information

Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

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Product References

Immunotherapy-resistant acute lymphoblastic leukemia cells exhibit reduced CD19 and CD22 expression and BTK pathway dependency.

The Journal of clinical investigation

Aminov S,Giricz O,Melnekoff DT,Sica RA,Polishchuk V,Papazoglu C,Yates B,Wang HW,Sahu S,Wang Y,Gordon-Mitchell S,Leshchenko VV,Schinke C,Pradhan K,Aluri S,Sohn M,Barta SK,Agarwal B,Goldfinger M,Mantzaris I,Shastri A,Matsui W,Steidl U,Brody JD,Shah NN,Parekh S,Verma A

Published figure using CD19 monoclonal antibody (Product # MHCD1928) in Flow Cytometry

Tue Feb 20 00:00:00 UTC 2024

Ex Vivo and In Vitro Methods for Detection of Bioactive Staphylococcal Enterotoxins.

Methods in molecular biology (Clifton, N.J.)

Rasooly R,Do P,Hernlem B

MHCD1928 was used in Flow cytometry/Cell sorting to investigate detection methods for Staphylococcal enterotoxins.

Wed Jan 19 00:00:00 UTC 2022

Prior infection with SARS-CoV-2 boosts and broadens Ad26.COV2.S immunogenicity in a variant-dependent manner.

Cell host & microbe

Keeton R,Richardson SI,Moyo-Gwete T,Hermanus T,Tincho MB,Benede N,Manamela NP,Baguma R,Makhado Z,Ngomti A,Motlou T,Mennen M,Chinhoyi L,Skelem S,Maboreke H,Doolabh D,Iranzadeh A,Otter AD,Brooks T,Noursadeghi M,Moon JC,Grifoni A,Weiskopf D,Sette A,Blackburn J,Hsiao NY,Williamson C,Riou C,Goga A,Garrett N,Bekker LG,Gray G,Ntusi NAB,Moore PL,Burgers WA

MHCD1928 was used in Flow Cytometry to compare participants who were SARS-CoV-2 naive with those either infected with the ancestral D614G virus or infected in the second wave when Beta predominated.

Wed Nov 10 00:00:00 UTC 2021

Senolytic CAR T cells reverse senescence-associated pathologies.

Nature

Amor C,Feucht J,Leibold J,Ho YJ,Zhu C,Alonso-Curbelo D,Mansilla-Soto J,Boyer JA,Li X,Giavridis T,Kulick A,Houlihan S,Peerschke E,Friedman SL,Ponomarev V,Piersigilli A,Sadelain M,Lowe SW

Published figure using CD19 monoclonal antibody (Product # MHCD1928) in Flow Cytometry

Wed Jul 01 00:00:00 UTC 2020

Senolytic CAR T cells reverse senescence-associated pathologies.

Nature

Amor C,Feucht J,Leibold J,Ho YJ,Zhu C,Alonso-Curbelo D,Mansilla-Soto J,Boyer JA,Li X,Giavridis T,Kulick A,Houlihan S,Peerschke E,Friedman SL,Ponomarev V,Piersigilli A,Sadelain M,Lowe SW

Published figure using CD19 monoclonal antibody (Product # MHCD1928) in Flow Cytometry

Wed Jul 01 00:00:00 UTC 2020

GAD-alum immunotherapy in type 1 diabetes expands bifunctional Th1/Th2 autoreactive CD4 T cells.

Diabetologia

Arif S,Gomez-Tourino I,Kamra Y,Pujol-Autonell I,Hanton E,Tree T,Melandri D,Hull C,Wherrett DK,Beam C,Roep BO,Lorenc A,Peakman M

Published figure using CD19 monoclonal antibody (Product # MHCD1928) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

GAD-alum immunotherapy in type 1 diabetes expands bifunctional Th1/Th2 autoreactive CD4 T cells.

Diabetologia

Arif S,Gomez-Tourino I,Kamra Y,Pujol-Autonell I,Hanton E,Tree T,Melandri D,Hull C,Wherrett DK,Beam C,Roep BO,Lorenc A,Peakman M

Published figure using CD19 monoclonal antibody (Product # MHCD1928) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

Principles Governing Establishment versus Collapse of HIV-1 Cellular Spread.

Cell host & microbe

Hataye JM,Casazza JP,Best K,Liang CJ,Immonen TT,Ambrozak DR,Darko S,Henry AR,Laboune F,Maldarelli F,Douek DC,Hengartner NW,Yamamoto T,Keele BF,Perelson AS,Koup RA

MHCD1928 was used in Flow Cytometry to explore the mechanism of exponential viral spreading of HIV-1 between cells.

Wed Dec 11 00:00:00 UTC 2019

Over-expression of PD-1 Does Not Predict Leukemic Relapse after Allogeneic Stem Cell Transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Jain P,Tian X,Cordes S,Chen J,Cantilena CR,Bradley C,Panjwani R,Chinian F,Keyvanfar K,Battiwalla M,Muranski P,Barrett AJ,Ito S

MHCD1928 was used in Flow cytometry/Cell sorting to analyse the relationship of leukaemia relapse with T-cell marker kinetics.

Fri Feb 01 00:00:00 UTC 2019

Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell.

Nature medicine

Ruella M,Xu J,Barrett DM,Fraietta JA,Reich TJ,Ambrose DE,Klichinsky M,Shestova O,Patel PR,Kulikovskaya I,Nazimuddin F,Bhoj VG,Orlando EJ,Fry TJ,Bitter H,Maude SL,Levine BL,Nobles CL,Bushman FD,Young RM,Scholler J,Gill SI,June CH,Grupp SA,Lacey SF,Melenhorst JJ

MHCD1928 was used in Flow cytometry/Cell sorting to report a patient relapsing 9 months after CD19-targeted CAR T cell (CTL019) infusion with CD19- leukemia that aberrantly expressed the anti-CD19 CAR.

Mon Oct 01 00:00:00 UTC 2018

Aggressive natural killer-cell leukemia mutational landscape and drug profiling highlight JAK-STAT signaling as therapeutic target.

Nature communications

Dufva O,Kankainen M,Kelkka T,Sekiguchi N,Awad SA,Eldfors S,Yadav B,Kuusanmäki H,Malani D,Andersson EI,Pietarinen P,Saikko L,Kovanen PE,Ojala T,Lee DA,Loughran TP,Nakazawa H,Suzumiya J,Suzuki R,Ko YH,Kim WS,Chuang SS,Aittokallio T,Chan WC,Ohshima K,Ishida F,Mustjoki S

MHCD1928 was used in Flow cytometry/Cell sorting to utilise genomic and drug sensitivity profiling to uncover the molecular pathogenesis of aggressive natural killer-cell leukemia.

Thu Apr 19 00:00:00 UTC 2018

Effect of Antiretroviral Therapy on the Memory and Activation Profiles of B Cells in HIV-Infected African Women.

Journal of immunology (Baltimore, Md. : 1950)

Tanko RF,Soares AP,Müller TL,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Riou C,Burgers WA

MHCD1928 was used in Flow cytometry/Cell sorting to study the effect of antiretroviral therapy on B cells in HIV infection.

Wed Feb 01 00:00:00 UTC 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

MHCD1928 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 UTC 2017

T cell receptor β-chains display abnormal shortening and repertoire sharing in type 1 diabetes.

Nature communications

Gomez-Tourino I,Kamra Y,Baptista R,Lorenc A,Peakman M

MHCD1928 was used in Flow cytometry/Cell sorting to suggest that short CDR3s increase the potential for self-recognition, conferring heightened risk of autoimmune disease.

Mon Nov 27 00:00:00 UTC 2017

Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a.

Clinical immunology (Orlando, Fla.)

Wahid R,Fresnay S,Levine MM,Sztein MB

MHCD1928 was used in flow cytometry to determine the S. Typhi, S. Paratyphi A, and S. Paratyphi B cross-reactive CD4 positive T cell responses elicited by immunization with Ty21a

Thu Dec 01 00:00:00 UTC 2016

Immunization with Ty21a live oral typhoid vaccine elicits crossreactive multifunctional CD8+ T-cell responses against Salmonella enterica serovar Typhi, S. Paratyphi A, and S. Paratyphi B in humans.

Mucosal immunology

Wahid R,Fresnay S,Levine MM,Sztein MB

MHCD1928 was used in Flow Cytometry to suggest that Ty21a elicits multifunctional CMI responses against Salmonella that could be critical in clearing the infection.

Sun Nov 01 00:00:00 UTC 2015

Long term maintenance of myeloid leukemic stem cells cultured with unrelated human mesenchymal stromal cells.

Stem cell research

Ito S,Barrett AJ,Dutra A,Pak E,Miner S,Keyvanfar K,Hensel NF,Rezvani K,Muranski P,Liu P,Larochelle A,Melenhorst JJ

MHCD1928 was used in flow cytometry to test if mesenchymal stromal cells support the growth and survival of leukemic stem cells in vitro.

Thu Jan 01 00:00:00 UTC 2015

Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-α monotherapy.

PloS one

Ilander M,Kreutzman A,Rohon P,Melo T,Faber E,Porkka K,Vakkila J,Mustjoki S

MHCD1928 was used in flow cytometry to examine the function of NK- and T-cells in order to understand how chronic myeloid leukemia patients maintain the treatment response after IFN-α discontinuation.

Wed Oct 15 00:00:00 UTC 2014

Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.

Clinical immunology (Orlando, Fla.)

McArthur MA,Sztein MB

MHCD1928 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Staphylococcal enterotoxin B and other superantigens cause toxic shock syndrome.

Fri Feb 01 00:00:00 UTC 2013

Quantitative and qualitative differences in the T cell response to HIV in uninfected Ugandans exposed or unexposed to HIV-infected partners.

Journal of virology

Pala P,Serwanga J,Watera C,Ritchie AJ,Moodie Z,Wang M,Goonetilleke N,Birabwa E,Hughes P,Senkaali D,Nakiboneka R,Grosskurth H,Haynes B,McMichael A,Kaleebu P

MHCD1928 was used in flow cytometry to characterize T cell responses of HIV-exposed, yet persistently uninfected individuals.

Thu Aug 01 00:00:00 UTC 2013

CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier.

Science translational medicine

Man S,Tucky B,Cotleur A,Drazba J,Takeshita Y,Ransohoff RM

MHCD1928 was used in flow cytometry to investigate leukocyte-endothelial interactions at the blood brain barrier

Wed Feb 01 00:00:00 UTC 2012

Unique biomarkers for B-cell function predict the serum response to pandemic H1N1 influenza vaccine.

International immunology

Frasca D,Diaz A,Romero M,Phillips M,Mendez NV,Landin AM,Blomberg BB

MHCD1928 was used in flow cytometry to assess the response to the pandemic (p)HN vaccine in young and elderly individuals in vivo and in vitro.

Thu Mar 01 00:00:00 UTC 2012

Reduced T regulatory cell response during acute Plasmodium falciparum infection in Malian children co-infected with Schistosoma haematobium.

PloS one

Lyke KE,Dabo A,Arama C,Daou M,Diarra I,Wang A,Plowe CV,Doumbo OK,Sztein MB

MHCD1928 was used in flow cytometry to discuss how co-infections and secondary parasite infections may disrupt the immunologic responses induced by a pre-existing parasitic infection.

Tue Jul 31 00:00:00 UTC 2012

Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.

PloS one

McArthur MA,Sztein MB

MHCD1928 was used in flow cytometry to explore the multifunctional IL-17A responses against S. Typhi antigens in T memory subsets.

Tue Oct 02 00:00:00 UTC 2012

Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

Berthoud TK,Hamill M,Lillie PJ,Hwenda L,Collins KA,Ewer KJ,Milicic A,Poyntz HC,Lambe T,Fletcher HA,Hill AV,Gilbert SC

MHCD1928 was used in flow cytometry to discuss the use of internal antigens to provide better protection against multiple influenza subtypes.

Sat Jan 01 00:00:00 UTC 2011

Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L

MHCD1928 was used in flow cytometry to test if anti-CD8 Abs induce effector function.

Fri Jul 15 00:00:00 UTC 2011

Immune modulation through 4-1BB enhances SIV vaccine protection in non-human primates against SIVmac251 challenge.

PloS one

Hirao LA,Hokey DA,Morrow MP,Jure-Kunkel MN,Weiner DB

MHCD1928 was used in flow cytometry to investigate the contribution of costimulatory molecules during SIV vaccination.

Tue Feb 28 00:00:00 UTC 2012

Intrinsic defects in B cell response to seasonal influenza vaccination in elderly humans.

Vaccine

Frasca D,Diaz A,Romero M,Landin AM,Phillips M,Lechner SC,Ryan JG,Blomberg BB

MHCD1928 was used in Flow cytometry/Cell sorting to demonstrate that age-related intrinsic B cell defects contribute to reduced antibody response to the influenza vaccine.

Mon Nov 29 00:00:00 UTC 2010

Activation drives PD-1 expression during vaccine-specific proliferation and following lentiviral infection in macaques.

European journal of immunology

Hokey DA,Johnson FB,Smith J,Weber JL,Yan J,Hirao L,Boyer JD,Lewis MG,Makedonas G,Betts MR,Weiner DB

MHCD1928 was used in Flow cytometry/Cell sorting to evaluate PD-1 expression during a primary immune response and persistent immune activation in macaques.

Thu May 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

Gene Aliases: B4; CD19; CVID3

UniProt ID: (Human) P15391

Entrez Gene ID: (Human) 930

Function(s)

Process(es)

cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response B cell receptor signaling pathway

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CD19 Monoclonal Antibody (SJ25-C1), Pacific Blue™

CD19 Antibody (MHCD1928) in Flow

Product Details

MHCD1928

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions