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Invitrogen

CD19 Monoclonal Antibody (SJ25C1), PE-Cyanine7, eBioscience™

Name: CD19 Monoclonal Antibody (SJ25C1), PE-Cyanine7, eBioscience™
Brand: Invitrogen
SKU: 25-0198-42
Price: 358.0 USD
Availability: InStock

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9 Published Figures

17 References

View all (353) CD19 antibodies

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Catalog # 25-0198-42

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Application

FIGURE: 1 / 11

CD19 Antibody (25-0198-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human CD19 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD19 Antibody — Antibody clone (SJ25C1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal was observed for target protein in CD19 KO cells (blue histogram) compared to the control Cas9 cells (pink histogram) using CD19 antibody (SJ25C1). Yellow histogram represents staining with the isotype control. {KO}

Product Details

25-0198-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

SJ25C1

Conjugate

PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10671548

Product Specific Information

Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.

Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Immune response induced by standard and fractional doses of 17DD yellow fever vaccine.

NPJ vaccines

Abdala-Torres T,Campi-Azevedo AC,da Silva-Pereira RA,Dos Santos LI,Henriques PM,Costa-Rocha IA,Otta DA,Peruhype-Magalhães V,Teixeira-Carvalho A,Araújo MSS,Fernandes EG,Sato HK,Fantinato FFST,Domingues CMAS,Kallás EG,Tomiyama HTI,Lemos JAC,Coelho-Dos-Reis JG,de Lima SMB,Schwarcz WD,de Souza Azevedo A,Trindade GF,Ano Bom APD,da Silva AMV,Fernandes CB,Camacho LAB,de Sousa Maia ML,Martins-Filho OA,do Antonelli LRDV

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Fri Mar 08 00:00:00 UTC 2024

Tyrosine Kinase Inhibitors Target B Lymphocytes.

Biomolecules

Upfold NLE,Petakh P,Kamyshnyi A,Oksenych V

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Sat Feb 25 00:00:00 UTC 2023

Evaluation of peripheral lymphocyte subsets in acne vulgaris patients before and after systemic isotretinoin treatment.

Indian journal of pharmacology

Gunaydin SD,Tezcan I

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Wed Dec 21 00:00:00 UTC 2022

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Cancer discovery

Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W

25-0198-42 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.

Thu Jul 01 00:00:00 UTC 2021

Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Nature communications

Asano Y,Daccache J,Jain D,Ko K,Kinloch A,Veselits M,Wolfgeher D,Chang A,Josephson M,Cunningham P,Tambur A,Khan AA,Pillai S,Chong AS,Clark MR

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Fri Jul 16 00:00:00 UTC 2021

Human chimeric antigen receptor macrophages for cancer immunotherapy.

Nature biotechnology

Klichinsky M,Ruella M,Shestova O,Lu XM,Best A,Zeeman M,Schmierer M,Gabrusiewicz K,Anderson NR,Petty NE,Cummins KD,Shen F,Shan X,Veliz K,Blouch K,Yashiro-Ohtani Y,Kenderian SS,Kim MY,O'Connor RS,Wallace SR,Kozlowski MS,Marchione DM,Shestov M,Garcia BA,June CH,Gill S

25-0198-42 was used in Flow Cytometry to show that in humanized mouse models, CAR-Ms were further shown to induce a pro-inflammatory tumor microenvironment and boost anti-tumor T cell activity.

Sat Aug 01 00:00:00 UTC 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Tue Apr 28 00:00:00 UTC 2020

The downregulated membrane expression of CD18 in CD34⁺ cells defines a primitive population of human hematopoietic stem cells.

Stem cell research & therapy

Mesa-Núñez C,Leon-Rico D,Aldea M,Damián C,Sanchez-Baltasar R,Sanchez R,Alberquilla O,Segovia JC,Bueren JA,Almarza E

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Tue Apr 28 00:00:00 UTC 2020

Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies.

JCI insight

Shah HB,Smith K,Scott EJ,Larabee JL,James JA,Ballard JD,Lang ML

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Thu Aug 20 00:00:00 UTC 2020

Identifying the Pathological Domain of Alpha- Synuclein as a Therapeutic for Parkinson's Disease.

International journal of molecular sciences

Shen N,Song G,Yang H,Lin X,Brown B,Hong Y,Cai J,Cao C

25-0198-42 was used in Flow Cytometry to identify the most potent therapeutic domain of alpha synuclein as a therapeutic target.

Sat May 11 00:00:00 UTC 2019

Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation.

The Journal of experimental medicine

Carpier JM,Zucchetti AE,Bataille L,Dogniaux S,Shafaq-Zadah M,Bardin S,Lucchino M,Maurin M,Joannas LD,Magalhaes JG,Johannes L,Galli T,Goud B,Hivroz C

25-0198 was used in Flow cytometry/Cell sorting to study the retrograde traffic of linker for activation of T cells from the plasma membrane to the Golgi-trans-Golgi network.

Mon Apr 02 00:00:00 UTC 2018

Efficient Non-viral Gene Delivery into Human Hematopoietic Stem Cells by Minicircle Sleeping Beauty Transposon Vectors.

Molecular therapy : the journal of the American Society of Gene Therapy

Holstein M,Mesa-Nuñez C,Miskey C,Almarza E,Poletti V,Schmeer M,Grueso E,Ordóñez Flores JC,Kobelt D,Walther W,Aneja MK,Geiger J,Bonig HB,Izsvák Z,Schleef M,Rudolph C,Mavilio F,Bueren JA,Guenechea G,Ivics Z

25-0198 was used in Flow cytometry/Cell sorting to improve Sleeping Beauty gene transfer into hard-to-transfect human CD34+ cells.

Wed Apr 04 00:00:00 UTC 2018

Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Dai J,Luftig MA

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Thu Mar 01 00:00:00 UTC 2018

Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Dai J,Luftig MA

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Thu Mar 01 00:00:00 UTC 2018

ILC2-modulated T cell-to-MDSC balance is associated with bladder cancer recurrence.

The Journal of clinical investigation

Chevalier MF,Trabanelli S,Racle J,Salomé B,Cesson V,Gharbi D,Bohner P,Domingos-Pereira S,Dartiguenave F,Fritschi AS,Speiser DE,Rentsch CA,Gfeller D,Jichlinski P,Nardelli-Haefliger D,Jandus C,Derré L

25-0198 was used in Flow cytometry/Cell sorting to underline the ILC2/IL-13 axis as a targetable pathway to curtail the M-MDSC compartment and improve bladder cancer treatment.

Tue Aug 01 00:00:00 UTC 2017

Antibody-Dependent NK Cell Activation Differentially Targets EBV-Infected Cells in Lytic Cycle and Bystander B Lymphocytes Bound to Viral Antigen-Containing Particles.

Journal of immunology (Baltimore, Md. : 1950)

López-Montañés M,Alari-Pahissa E,Sintes J,Martínez-Rodríguez JE,Muntasell A,López-Botet M

25-0198 was used in Flow cytometry/Cell sorting to support that Ab-dependent NK cell activation plays an important role in the control of EBV, enhancing NK cell effector functions against infected B cells in the lytic cycle.

Sat Jul 15 00:00:00 UTC 2017

The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.

Scandinavian journal of immunology

Albayati Z,Alyami A,Alomar S,Middleton D,Bonnett L,Aleem S,Flanagan BF,Christmas SE

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Wed Nov 01 00:00:00 UTC 2017

Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.

European journal of immunology

Ndungu FM,Lundblom K,Rono J,Illingworth J,Eriksson S,Färnert A

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Fri Nov 01 00:00:00 UTC 2013

Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.

Journal of immunology (Baltimore, Md. : 1950)

Illingworth J,Butler NS,Roetynck S,Mwacharo J,Pierce SK,Bejon P,Crompton PD,Marsh K,Ndungu FM

Published figure using CD19 monoclonal antibody (Product # 25-0198-42) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19; Differentiation antigen CD19; Leu-12; T-cell surface antigen Leu-12

Gene Aliases: B4; CD19; CVID3

UniProt ID: (Human) P15391

Entrez Gene ID: (Human) 930

Function(s)

Process(es)

cellular defense response cell surface receptor signaling pathway regulation of phosphatidylinositol 3-kinase signaling phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling regulation of immune response B cell receptor signaling pathway

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Learn more

CD19 Monoclonal Antibody (SJ25C1), PE-Cyanine7, eBioscience™

CD19 Antibody (25-0198-42) in Flow

Product Details

25-0198-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions