Invitrogen

CD1d Monoclonal Antibody (1B1), eBioscience™

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Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD1d Antibody (14-0011-82) in Flow

Staining of BALB/c splenocytes with 1.0 µg of Rat IgG2b Isotype Control Purified (Product # 14-4031-82) (open histogram) or 1.0 µg of Anti-Mouse CD1d Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Total viable cells were used for analysis.

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Product Details

14-0011-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-Dependent

Flow Cytometry (Flow)

1 µg/test

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1B1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467036

Product Specific Information

Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.

Applications Reported: The 1B1 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 1B1 has also been reported in in vivo and in vitro studies. (Please use Functional Grade purified 1B1, Product # 16-0011, in functional assays).

Applications Tested: The 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Flow Cytometry

Fri Feb 11 00:00:00 EST 2022

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

FHL1 reduces dystrophy in transgenic mice overexpressing FSHD muscular dystrophy region gene 1 (FRG1).

PloS one

Feeney SJ,McGrath MJ,Sriratana A,Gehrig SM,Lynch GS,D'Arcy CE,Price JT,McLean CA,Tupler R,Mitchell CA

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Western Blot

Mon Nov 16 00:00:00 EST 2015

iNKT cell cytotoxic responses control T-lymphoma growth in vitro and in vivo .

Cancer immunology research

Bassiri H,Das R,Guan P,Barrett DM,Brennan PJ,Banerjee PP,Wiener SJ,Orange JS,Brenner MB,Grupp SA,Nichols KE

14-0011 was used in Functional assays to investigate the contribution of iNKT cells to tumour immune surveillance.

Wed Jan 01 00:00:00 EST 2014

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD1d monoclonal antibody (Product # 14-0011-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Preactivation of NKT cells with alpha-GalCer protects against hepatic ischemia-reperfusion injury in mouse by a mechanism involving IL-13 and adenosine A2A receptor.

American journal of physiology. Gastrointestinal and liver physiology

Cao Z,Yuan Y,Jeyabalan G,Du Q,Tsung A,Geller DA,Billiar TR

14-0011 was used in Blocking experiments to investigate whether preactivation of NKT cells could provide a beneficial "preconditioning" effect to ameliorate subsequent hepatic ischemia-reperfusion injury.

Sat Aug 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Antigen-presenting glycoprotein CD1d1; CD1d.1; T-cell surface glycoprotein CD1d1

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Gene Aliases: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Ly-38

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UniProt ID: (Mouse) P11609

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Entrez Gene ID: (Mouse) 12479

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Function(s)

protein binding beta-2-microglobulin binding lipid antigen binding endogenous lipid antigen binding exogenous lipid antigen binding histone binding T cell receptor binding cell adhesion molecule binding lipopeptide binding major histocompatibility complex protein

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD1d Monoclonal Antibody (1B1), eBioscience™

CD1d Antibody (14-0011-82) in Flow

Product Details

14-0011-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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